| 货号 | AF2929-SP |
| 别名 | FLJ27515; Smuckler; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell membrane protein 4; TIM4; TIM-4; TIMD4; TIMD-4 | 全称 | T Cell Immunoglobulin Mucin-4 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human TIM-4 in direct ELISAs and Western blots. In these formats, less than 1% cross‑reactivity with recombinant human (rh) TIM-1, recombinant mouse (rm) TIM-2, rhTIM-3, rmTIM-4, rmTIM-5, and rmTIM-6 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 91937 (Human); 276891 (Mouse); 102140672 (Cynomolgus Monkey) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Characterizing functional domains for TIM-mediated enveloped virus entry. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human TIM-4 (R&D Systems, Catalog # 2929-TM) Glu25-Leu315 Accession # Q96H15 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1 - 3). The human TIM-4 cDNA encodes a 378 amino acid (aa) precursor that includes a 24 aa signal sequence, a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1 or TIM-3. Within the ECD, human TIM-4 shares 35% and 23% aa sequence identity with TIM-1 and TIM-3, respectively. A TIM-2 ortholog has not been identified in human. The ECD of human TIM-4 shares 45% aa sequence identity with that of mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma (5 - 7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells (5, 8). The interaction of TIM-4 with TIM-1 induces costimulatory and hyperproliferative signals in T cells (5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Recombinant Human TIM‑4 by Western Blot. Western blot shows 25 ng of Recombinant Human TIM‑4 His-tag (Catalog # 2929-TM), Recombinant Human TIM‑1/KIM‑1/HAVCR (Catalog # 1750-TM), Recombinant Human TIM‑3 Fc Chimera (Catalog # 2365-TM), Recombinant Mouse TIM‑1/KIM‑1/HAVCR (Catalog # 1817-TM), Recombinant Mouse TIM‑2 (Catalog # 1885-TM), Recombinant Mouse TIM‑3 Fc Chimera (Catalog # 1529-TM), Recombinant Mouse TIM‑4 (Catalog # 2826-TI), Recombinant Mouse TIM-5, and Recombinant Mouse TIM-6. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human TIM‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2929) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TIM‑4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. |
