| 货号 | AF2928-SP |
| 别名 | MYD88D; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88 | 全称 | Myeloid Differentiation Primary Response Gene 88 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) Immunocytochemistry(5-15 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human MyD88 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse MyD88 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL Immunocytochemistry: 5-15 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4615 (Human); 17874 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Potentiation of flagellin responses in gut epithelial cells by interferon-gamma is associated with STAT-independent regulation of MyD88 expression. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MyD88 Met1-Pro296 Accession # Q99836 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Myeloid Differentiation primary response gene 88 (MyD88) is a 35 kDa adaptor protein involved in the IL-1 signaling pathway and recruits IRAK-2 to the IL-1 receptor complex. MyD88 also recruits IRAK-4 to Toll-like receptors 2 and 4 (TLR2 and TLR4) and plays an important role in the inflammatory response induced by IL-1, IL-18 and endotoxin. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human MyD88 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line, Jurkat human acute T cell leukemia cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human MyD88 (1 ng) was included. A specific band for MyD88 was detected at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. | |
MyD88 in Raji Human Cell Line. MyD88 was detected in immersion fixed Raji human Burkitts lymphoma cell line using Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. | |
Detection of MyD88 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitts lymphoma cell line was stained with Goat Anti-Human MyD88 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2928, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. | |
Detection of Human MyD88 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitts lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MyD88 at approximately 41 kDa (as indicated) using 5 µg/mL of Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
