Human MIF Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human MIF Affinity Purified Polyclonal Ab (25 UG)

货号： AF-289-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-289-SP
别名	EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase
全称	Macrophage Migration Inhibitory Factor
反应种属	Human
应用	Western Blot,CyTOF-ready,Intracellular Staining by Flow Cytometry
目标/特异性	Detects human, mouse, and rat MIF in Western blots.
使用方法	Western Blot: 0.5 µg/mL CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Intracellular Staining by Flow Cytometry: 0.25 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4282 (Human); 17319 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells. Authors: DiCosmo-Ponticello C, Hoover D, Coffman F, Cohen S, Cohen M Cytokine, 2014;69(1):47-55. Species: Human Sample Type: Cell Lysates Application: WB Effect of macrophage migration inhibitory factor (MIF) in human placental explants infected with Toxoplasma gondii depends on gestational age. Authors: de Oliveira Gomes A, de Oliveira Silva DA, Silva NM, de Freitas Barbosa B, Franco PS, Angeloni MB, Fermino ML, Roque-Barreira MC, Bechi N, Paulesu LR, Dos Santos MC, Mineo JR, Ferro EA Am. J. Pathol., 2012;178(6):2792-801. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Human liver stem cell-derived microvesicles inhibit hepatoma growth in SCID mice by delivering antitumor microRNAs. Authors: Fonsato V, Collino F, Herrera M, Cavallari C, Deregibus M, Cisterna B, Bruno S, Romagnoli R, Salizzoni M, Tetta C, Camussi G Stem Cells, 2012;30(9):1985-98. Species: Human Sample Type: Whole Cells Application: IHC Paraffin-embedded Critical role for macrophage migration inhibitory factor (MIF) in Ross River virus-induced arthritis and myositis. Authors: Herrero LJ, Nelson M, Srikiatkhachorn A, Gu R, Anantapreecha S, Fingerle-Rowson G, Bucala R, Morand E, Santos LL, Mahalingam S Proc. Natl. Acad. Sci. U.S.A., 2011;108(29):12048-53. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Macrophage migration inhibitory factor anti-thrombin III complexes are decreased in bladder cancer patient serum: Complex formation as a mechanism of inactivation. Authors: Meyer-Siegler KL, Cox J, Leng L, Bucala R, Vera PL Cancer Lett., 2010;290(1):49-57. Species: Human Sample Type: Serum Application: WB Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells. Authors: Ansa-Addo EA, Lange S, Stratton D, Antwi-Baffour S, Cestari I, Ramirez MI, McCrossan MV, Inal JM J. Immunol., 2010;185(9):5236-46. Species: Human Sample Type: Whole Cells Application: Flow Sex dimorphism in wound healing: the roles of sex steroids and macrophage migration inhibitory factor. Authors: Gilliver SC, Ruckshanthi JP, Hardman MJ, Nakayama T, Ashcroft GS Endocrinology, 2008;149(11):5747-57. Species: Mouse Sample Type: Tissue Homogenates Application: WB Apoptotic neutrophils release macrophage migration inhibitory factor upon stimulation with tumor necrosis factor-alpha. Authors: Daryadel A, Grifone RF, Simon HU, Yousefi S J. Biol. Chem., 2006;281(37):27653-61. Species: Human Sample Type: Cell Lysates Application: WB Variation in macrophage-migration-inhibitory-factor immunoreactivity during porcine gestation. Authors: Paulesu L, Cateni C, Romagnoli R, Ietta F, Dantzer V Biol. Reprod., 2005;72(4):949-53. Species: Human Sample Type: Tissue Homogenates Application: WB High concentrations of circulating macrophage migration inhibitory factor in patients with severe blunt trauma: Is serum macrophage migration inhibitory factor concentration a valuable prognostic factor? Authors: Chuang CC, Hung CJ, Tsai MC, Chuang YC Crit. Care Med., 2004;32(3):734-9. Species: Human Sample Type: Serum Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human MIF Pro2-Ala115 Accession # AAA36315
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4-7). Secretion occurs non-classically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50-65 have also been suggested to contain thiol-protein oxidoreductase activity (12). MIF has proinflammatory cytokine activity centered around aa’s 49-65. On fibroblasts, MIF induces, IL-1, IL-8, and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release following IFN-gamma activation (13, 14). MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (15, 16). Human MIF is active on mouse cells (14). Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine, and rat MIF, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Norand, E.F. and M. Leech (2005) Front. Biosci. 10:12. Donn, R.P. and D.W. Ray (2004) J. Endocrinol. 182:1. Calandra, T. and T. Roger (2003) Nat. Rev. Immunol. 3:791. Kozak, C.A. et al. (1995) Genomics 27:405. Weiser, W.Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7522. Paralkar, V. and G. Wistow (1994) Genomics 19:48. Wistow, G.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1272. Flieger, O. et al. (2003) FEBS Lett. 551:78. Philo, J.S. et al. (2004) Biophys. Chem. 108:77. Sun, H-W. et al. (1996) Protein Eng. 9:631. Stamps, S.L. et. al. (2000) Biochemistry 39:9671. Nguyen, M.T. et al. (2003) J. Biol. Chem. 278:33654. Sato, A. et al. (2003) Dev. Comp. Immunol. 27:401. Bernhagen, J. et al. (1994) Biochemistry 33:14144. Leng, L. et al. (2003) J. Exp. Med. 197:1467. Meyer-Siegler, K.L. and P.L. Vera (2005) J. Urol. 173:615.

参考图片

Detection of Human MIF by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat MIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-289-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse and Rat MIF by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat MIF Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF-289-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MIF at approximately 12-14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.