| 货号 | AF2889-SP |
| 别名 | B-cell CLL/lymphoma 10CIPERCLAPB-cell lymphoma/leukemia 10; Bcl-10; CARD-containing apoptotic signaling protein; CARD-containing molecule enhancing NF-kappa-B; CARD-containing proapoptotic protein; CARD-like apoptotic protein; CARMEN; caspase-recruiting domain-containing protein; cCARMEN; c-E10; c-E10bcl-10; CED-3/ICH-1 prodomain homologous E10-like regulator; Cellular homolog of vCARMEN; cellular-E10; CIPER; CLAP; hCLAP; Mammalian CARD-containing adapter molecule E10; mE10; mE10CARD containing molecule enhancing NF-kB | 全称 | B Cell Lymphoma/Leukemia 10 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Bcl-10 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8915 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Bcl-10 Met1-Leu192 Accession # O95999 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | B cell CLL/leukemia 10 (Bcl-10), a 233 amino acid, 26.2 kDa protein, is the cellular homolog of the equine herpesvirus-2 gene (E10). Bcl-10 is also known as CARD like apoptotic protein (CLAP), CED-3/ICH-1 prodomain homologous E10-like regulator (CIPER), celluar-E10 (c-E10), and CARD-containing molecule enhancing NF kappa B (CARMEN). Bcl-10 contains a caspase recruitment domain (CARD) and has been shown to induce apoptosis and to activate NF kappa B. Bcl-10 can interact with other CARD domain containing proteins including CARD9, 10, 11, and 14, which function as upstream regulators of NF kappa B signaling. Defects in Bcl‑10 may be involved in the pathogenesis of human malignancy. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Bcl‑10 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line, A431 human epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Bcl‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2889) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Bcl-10 at approximately 28 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 4. | |
Bcl‑10 in A431 Human Cell Line. Bcl‑10 was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human Bcl‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2889) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
