| 货号 | AF2886-SP |
| 别名 | Basic-helix-loop-helix-PAS protein MOP2; BHLHE73; bHLHe73ECYT4; Class E basic helix-loop-helix protein 73; endothelial PAS domain protein 1; endothelial PAS domain-containing protein 1; EPAS1; EPAS-1; HIF-1alpha-like factor; HIF-2 alpha; hif2a angiogenesis; HIF2A; HIF2AHIF-2-alpha; HLF; HLFHIF-1-alpha-like factor; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; MOP2; MOP2HIF2-alpha; PASD2PAS domain-containing protein 2 | 全称 | Hypoxia-inducible Transcription Factor 2 alpha |
| 反应种属 | Human/Rat |
| 应用 | Western Blot(1 µg/mL) Chromatin Immunoprecipitation (ChIP)(5 µg/5 x 106cells) |
| 目标/特异性 | Detects human and rat HIF‑2 alpha /EPAS1. |
| 使用方法 | Western Blot: 1 µg/mL Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2034 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41 | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human HIF‑2 alpha /EPAS1 Ser543-Thr870 Accession # Q99814 |
| 生物活性 | Human, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The hypoxia-inducible transcription factor 2 alpha (HIF-2 alpha ) is stabilized in hypoxic tissue and, similarly to HIF-1 alpha, complexes with Aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear completely redundant, although specific functions are only beginning to be elucidated. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the potentially broad impact of gene regulation by both HIF-1 alpha and HIF-2 alpha. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Rat HIF-2 alpha /EPAS1 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line and PC‑12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl2 for 16 h. PVDF membrane was probed with 1 µg/mL of Human/Rat HIF‑2 alpha /EPAS1 Polyclonal Antibody, followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF‑2 alpha /EPAS1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of HIF‑2 alpha /EPAS1-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells treated with 150 μM CoCl2 overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HIF‑2 alpha /EPAS1/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Rat HIF‑2 alpha /EPAS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2886) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The oct-3/4 promoter was detected by standard PCR. |
