Human VEGF-D Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human VEGF-D Affinity Purified Polyclonal Ab (25 UG)

货号： AF286-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF286-SP
别名	c-fos induced growth factor (vascular endothelial growth factor D); FIGF; vascular endothelial growth factor D; VEGF-DVEGFDc-Fos-induced growth factor
全称	Vascular Endothelial Growth Factor D/cFos-induced Growth Factor
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human VEGF-D in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with mVEGF-D and less than 1% cross-reactivity with recombinant human (rh) VEGF‑C is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize VEGF‑D-induced proliferation in HMVEC human microvascular endothelial cells [Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548]. The Neutralization Dose (ND₅₀) is typically 3‑10 µg/mL in the presence of 1 µg/mL Recombinant Human VEGF‑D.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2277 (Human); 14205 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Dilated thin-walled blood and lymphatic vessels in human endometrium: a potential role for VEGF-D in progestin-induced break-through bleeding. Authors: Donoghue JF, McGavigan CJ, Lederman FL PLoS ONE, 2012;7(2):e30916. Species: Human Sample Type: Cell Lysates Application: WB Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1. Authors: Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, Loges S, Kilic E, Weil J, Lauke H, Tilki D, Singer BB, Ergun S Blood, 2007;110(13):4223-33. Species: Human Sample Type: Cell Lysates Application: WB Radiogenic lymphangiogenesis in the skin. Authors: Jackowski S, Janusch M, Fiedler E, Marsch WC, Ulbrich EJ, Gaisbauer G, Dunst J, Kerjaschki D, Helmbold P Am. J. Pathol., 2007;171(1):338-48. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Apoptosis of human macrophages by Flt-4 signaling: implications for atherosclerotic plaque pathology. Authors: Schmeisser A, Christoph M, Augstein A, Marquetant R, Kasper M, Braun-Dullaeus RC, Strasser RH Cardiovasc. Res., 2006;71(4):774-84. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded The expression pattern of vascular endothelial growth factor C and D in human esophageal normal mucosa, dysplasia and neoplasia. Authors: Ishikawa M, Kazama S Hepatogastroenterology, 2004;51(59):1319-22. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Cyclooxygenase-2 induces EP1- and HER-2/Neu-dependent vascular endothelial growth factor-C up-regulation: a novel mechanism of lymphangiogenesis in lung adenocarcinoma. Authors: Su JL, Shih JY, Yen ML, Jeng YM, Chang CC, Hsieh CY, Wei LH, Yang PC, Kuo ML Cancer Res., 2004;64(2):554-64. Species: Human Sample Type: Cell Lysates Application: WB Expression of vascular endothelial growth factor (VEGF)-D and its receptor, VEGF receptor 3, as a prognostic factor in endometrial carcinoma. Authors: Yokoyama Y, Charnock-Jones DS, Licence D, Yanaihara A, Hastings JM, Holland CM, Emoto M, Sakamoto A, Sakamoto T, Maruyama H, Sato S, Mizunuma H, Smith SK Clin. Cancer Res., 2003;9(4):1361-9. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human VEGF-D Phe93-Ser201 Accession # O43915
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Vascular endothelial growth factor D (VEGF-D), also known asc-fos-induced growth factor (FIGF), is a secreted glycoprotein of the VEGF/PDGF family. VEGFs regulate angiogenesis and lymphangiogenesis during development and tumor growth, and are characterized by eight conserved cysteine residues that form a cystine knot structure (1-3). VEGF-C and VEGF-D, which share 23% amino acid (aa) sequence identity, are uniquely expressed as preproproteins that contain long N- and C‑terminal propeptide extensions around the VEGF homology domain (VHD) (1, 2). Proteolytic processing of the 354 aa VEGF-D preproprotein creates a secreted proprotein. Further processing by extracellular serine proteases, such as plasmin or furin-like proprotein convertases, forms mature VEGF-D consisting of non-covalently linked 42 kDa homodimers of the 117 aa VHD (4-6). Mature human VEGF-D shares 94%, 95%, 99%, 97%, and 93% aa identity with mouse, rat, equine, canine and bovine VEGF-D, respectively (4, 5). It is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin (1-4). Mouse and human VEGF-D are ligands for VEGF Receptor 3 (VEGF R3; also called Flt-4) that are active across species and show enhanced affinity when processed (7). Processed human VEGF-D is also a ligand for VEGF R2, also called Flk-1 or KDR (7). VEGF R3 is strongly expressed in lymphatic endothelial cells and is essential for regulation of the growth and differentiation of lymphatic endothelium (1, 2). While VEGF-C is the critical ligand for VEGF R3 during embryonic lymphatic development, VEGF-D is most active in neonatal lymphatic maturation and bone growth (8-10). Both promote tumor lymphangiogenesis (11). Consonant with their activity on VEGF receptors, binding of VEGF-C and VEGF-D to neuropilins contributes to VEGF R3 signaling in lymphangiogenesis, while binding to integrin alpha 9 beta 1 mediates endothelial cell adhesion and migration (12, 13).
运输条件	Blue Ice
存放说明	4℃
参考文献	Roy, H. et al. (2006) FEBS Lett. 580:2879. Otrock, Z.H. et al. (2007) Blood Cells Mol. Dis. 38:258. Yamada, Y. et al. (1997) Genomics 42:483. Stacker, S.A. et al. (1999) J. Biol. Chem. 274:32127. McColl, B.K. et al. (2003) J. Exp. Med. 198:863. McColl, B.K. et al. (2007) FASEB J. 21:1088. Baldwin, M.E. et al. (2001) J. Biol. Chem. 276:19166. Baldwin, M.E. et al. (2005) Mol. Cell. Biol. 25:2441. Karpanen, T. et al. (2006) Am. J. Pathol. 169:708. Orlandini, M. et al. (2006) J. Biol. Chem. 281:17961. Stacker, S.A. et al. (2001) Nature Med. 7:186. Karpanen, T. et al. (2006) FASEB J. 20:1462. Vlahakis, N.E. et al. (2005) J. Biol. Chem. 280:4544.

参考图片

Cell Proliferation Induced by VEGF‑D and Neutralization by Human VEGF‑D Antibody. Recombinant Human VEGF‑D (Catalog # 622-VD) stimulates proliferation in HMVEC human microvascular endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF‑D (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGF-D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF286). The ND₅₀ is typically 3-10 µg/mL.

VEGF‑D in Human Prostate. VEGF‑D was detected in immersion fixed frozen sections of human prostate (cross-section through a blood vessel) using 15 µg/mL Goat Anti-Human VEGF‑D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF286) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.