Human IFN-gamma Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IFN-gamma Affinity Purified Polyclonal Ab (25 UG)

货号： AF-285-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-285-SP
别名	IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma
全称	Interferon gamma
反应种属	Human
应用	Western Blot,Immunocytochemistry,Neutralization
目标/特异性	Detects human IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant rat IFN-gamma and recombinant mouse IFN-gamma is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND₅₀) is typically 0.1-0.3 µg/mL in the presence of 5 ng/mL Recombinant Human IFN‑ gamma.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The Therapeutic Effect of Pamidronate on Lethal Avian Influenza A H7N9 Virus Infected Humanized Mice. Authors: Zheng J, Wu W, Liu Y, Xiang Z, Liu M, Chan K, Lau S, Lam K, To K, Chan J, Li L, Chen H, Lau Y, Yuen K, Tu W PLoS ONE, 2015;10(8):e0135999. Species: Human Sample Type: Whole Cells Application: Neut Immunoproteasome expression is induced in mesial temporal lobe epilepsy. Authors: Mishto M, Ligorio C, Bellavista E, Martucci M, Santoro A, Giulioni M, Marucci G, Franceschi C Biochem. Biophys. Res. Commun., 2011;408(1):65-70. Species: Human Sample Type: Tissue Homogenates Application: WB Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma. Authors: Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C PLoS Pathog., 2011;7(4):e1002016. Species: Human Sample Type: Whole Cells Application: Neut Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans. Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ Blood, 2010;116(12):2061-9. Species: Human Sample Type: Whole Tissue Application: IHC - Not specified IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients. Authors: Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK Immunol. Lett., 2010;127(2):150-6. Species: Human Sample Type: Whole Cells Application: Neut Evolving immunosuppressive microenvironment during human cervical carcinogenesis. Authors: Kobayashi A, Weinberg V, Darragh T, Smith-McCune K Mucosal Immunol, 2008;1(5):412-20. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Upregulated IL-23 and IL-17 in Behcet patients with active uveitis. Authors: Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A Invest. Ophthalmol. Vis. Sci., 2008;49(7):3058-64. Species: Human Sample Type: Whole Cells Application: Neut Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S Eur. J. Immunol., 2008;38(4):1012-23. Species: Human Sample Type: Whole Cells Application: Neut Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma. Authors: Hessian PA, Highton J, Kean A, Sun CK, Chin M Arthritis Rheum., 2003;48(2):334-8. Species: Human Sample Type: Whole Tissue Application: IHC Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1. Authors: Wang R, Jaw JJ, Jaw J, Stutzman N, Stutzman NC, Zou Z, Sun PD J Leukoc Biol, 0;91(2):299-309. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF) Met1-Gln144 Accession # Q14609
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.
运输条件	Blue Ice
存放说明	4℃
参考文献	Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines and Receptors in Tfh Cell Differentiation and Function gamma delta T Cells Group 1 ILCs IL-10/Interferon Family Infectious Diseases Inflammatory Mediators Isolation, Culture, and Identification of Natural Killer Cells Macrophage Activation Markers Molecules Secreted by VSMC Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) Natural Killer T (NKT) Cells NCR+ILC3 NCR-ILC3 Neuroinflammation Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th1 Cells

参考图片

IFN‑ gamma in Human PBMCs. IFN‑ gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Goat Anti-Human IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑285‑NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma Antibody. Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN‑ gamma (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA). The ND₅₀ is typically 0.1‑0.3 µg/mL.