| 货号 | AF2855-SP |
| 别名 | ERK activator kinase 2; MAP2K2; MAPKK 2; MEK 2; MEK2EC 2.7.12.2; mitogen-activated protein kinase kinase 2; MKK2; MKK2FLJ26075; p45; PRKMK2MAPKK2 | 全称 | Mitogen-activated Protein Kinase Kinase 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human MEK2 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5605 (Human); 26396 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MEK2 Met1-Val400 Accession # P36507 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mitogen-activated protein kinase kinase 2 (MAP2K2), also known as MEK2 and MKK2, belongs to the STE grouping of kinases. Both MEK2 and the related MEK1 are dual-specificity kinases, phosphorylating and activating the mitogen-activated protein kinases ERK1 and ERK2 at T and Y positions within the phosphoacceptor sequence T-E-Y. Activation of MEK2 by Raf occurs through phosphorylation at S222 and S226. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human MEK2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, Ramos human Burkitts lymphoma cell line, and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human MEK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2855) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human MEK1 and Recombinant Human Active MEK2 (Catalog # 2855-KS) (10 ng/lane) were included. A specific band for MEK2 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
