| 货号 | AF283-SP |
| 别名 | dJ380B8.2; neuritin 1; neuritin; NRN1; NRNMGC44811 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(0.25 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Neuritin in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 51299 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Neuritin Upregulates Kv4.2 ?-subunit of Potassium Channel Expression and Affects Neuronal Excitability by Regulating the Calcium-Calcineurin-NFATc4 Signaling Pathway | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Neuritin Ala28-Gly114 Accession # Q9NPD7 |
| 生物活性 | Human, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Neuritin is a 14 kDa GPI-linked molecule that is expressed on developing and differentiating neurons as well as on hepatocytes. Neuritin is upregulated in response to BDNF, NT-3, androgens, and hypoxia, and it promotes neurite extension and arborization. Mature human Neuritin shares 99% and 100% aa sequence identity with mouse and rat Neuritin, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Neuritin by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Neuritin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF283) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Neuritin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Neuritin in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Goat Anti-Human/Rat Neuritin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF283, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. |
