Human CCL7/MCP-3 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human CCL7/MCP-3 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-282-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-282-SP
别名	C-C motif chemokine 7; CCL7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3)
反应种属	Human
应用	Western Blot,Neutralization
目标/特异性	Detects human CCL7/MCP‑3/MARC in direct ELISAs and Western blots. In these formats, less than 20% cross-reactivity with recombinant human (rh) Eotaxin is observed and less than 5% cross-reactivity with rhMCP-2, rhMCP-1, and recombinant mouse JE is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CCL7/MCP‑3/MARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND₅₀) is typically 10-30 µg/mL in the presence of 1 µg/mL Recombinant Human CCL7/MCP‑3/MARC.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6354 (Human); 20306 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Evidence for chemokine synergy during neutrophil migration in ARDS Thorax, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human CCL7/MCP‑3/MARC (R&D Systems, Catalog # 282-P3) Gln34-Leu109 Accession # Q7Z7Q8
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2. CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites. Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4⁺ and CD8⁺ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
运输条件	Blue Ice
存放说明	4℃
参考文献	CC Chemokines and Receptors Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis Inflammatory Mediators Molecules Secreted by VSMC Th22 Cells

参考图片

Chemotaxis Induced by CCL7/MCP‑3 and Neutralization by Human CCL7/MCP‑3 Antibody. Recombinant Human CCL7/MCP‑3 (Catalog # 282-P3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL7/MCP‑3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL7/MCP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-282-NA). The ND₅₀ is typically 10-30 µg/mL.