Human IL-1ra/IL-1F3 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-1ra/IL-1F3 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-280-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-280-SP
别名	DIRA; ICIL-1RA; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4
全称	Interleukin 1 Receptor Antagonist
反应种属	Human
应用	Western Blot,Simple Western,Immunohistochemistry,Neutralization
目标/特异性	Detects human IL‑1ra/IL-1F3 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 15% cross-reactivity with recombinant mouse IL-1ra is observed.
使用方法	Western Blot: 0.5 µg/mL Simple Western: 20 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑1ra/IL‑1F3 inhibition of IL‑1 alpha /IL‑1F1-dependent proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically ≤4 µg/mL in the presence of 50 ng/mL Recombinant Human IL‑1ra/IL‑1F3 and 50 pg/mL Recombinant Human IL‑1 alpha /IL‑1F1.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Prostaglandin E2 Indicates Therapeutic Efficacy Of Mesenchymal Stem Cells In Experimental Traumatic Brain Injury Authors: DJ Kota, KS Prabhakara, N Toledano-F, D Bhattarai, Q Chen, B DiCarlo, P Smith, F Triolo, PL Wenzel, CS Cox, SD Olson Stem Cells, 2017;0(0):. Species: Human Sample Type: Cell Lysates Application: WB IL-1 Receptor Antagonist Chimeric Protein: Context-Specific and Inflammation-Restricted Activation. Authors: Rider P, Carmi Y, Yossef R, Guttman O, Eini H, Azam T, Dinarello C, Lewis E J Immunol, 2015;195(4):1705-12. Species: Human Sample Type: Cell Lysates Application: WB IL-4 enhances IFN-lambda1 (IL-29) production by plasmacytoid DCs via monocyte secretion of IL-1Ra. Authors: Megjugorac NJ, Gallagher GE, Gallagher G Blood, 2010;115(21):4185-90. Species: Human Sample Type: Whole Cells Application: Neut Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers. Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH Dig. Dis. Sci., 2007;52(10):2775-83. Species: Human Sample Type: Whole Cells Application: ICC Immunolocalization of interleukin-1 receptors in the sarcolemma and nuclei of skeletal muscle in patients with idiopathic inflammatory myopathies. Authors: Grundtman C, Salomonsson S, Dorph C, Bruton J, Andersson U, Lundberg IE Arthritis Rheum., 2007;56(2):674-87. Species: Human Sample Type: Whole Cells Application: ICC P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells. Authors: Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC Br. J. Pharmacol., 2007;151(1):115-27. Species: Human Sample Type: Cell Culture Supernates Application: WB IL-22 increases the innate immunity of tissues. Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R Immunity, 2004;21(2):241-54. Species: Human Sample Type: Whole Cells Application: Neut T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta. Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM J. Allergy Clin. Immunol., 2002;110(4):624-33. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IL‑1ra/IL-1F3 Arg26-Glu177 Accession # P18510
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitroandin vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cytokine and Growth Factor Receptors on VSMC Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis IL-1 Family Macrophage Activation Markers Neuroinflammation

参考图片

Detection of Human IL‑1ra/IL‑1F3 by Western Blot. Western blot shows lysates of RT-4 human bladder carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑1ra/IL‑1F3 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑1ra/IL‑1F3 in Human Skin. IL‑1ra/IL‑1F3 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human and Mouse IL‑1ra/IL‑1F3 by Simple Western^TM. Simple Western lane view shows lysates of RT-4 human bladder carcinoma cell line, A431 human epithelial carcinoma cell line, human skin cancer tissue, and mouse skin cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for IL‑1ra/IL‑1F3 at approximately 24-26 kDa (as indicated) using 20 µg/mL of Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

IL‑1ra/IL‑1F3 Inhibition of
IL‑1 alpha /IL‑1F1-dependent Cell Proliferation and Neutralization by Human
IL‑1ra/IL‑1F3 Antibody. Recombinant Human
IL‑1ra/IL‑1F3 (Catalog #
280‑RA) inhibits Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog #
200‑LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog #AR002). Inhibition of Recombinant Human
IL‑1 alpha /IL‑1F1 (50 pg/mL) activity elicited by Recombinant Human IL‑1ra/IL‑1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA). The ND₅₀ is typically ≤4 µg/mL.