Human Syndecan-1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Syndecan-1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF2780-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2780-SP
别名	CD138 antigen; CD138; SDC; SDC1; SYND1heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; syndecan proteoglycan 1; syndecan; syndecan-1
反应种属	Human
应用	Western Blot,Immunohistochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard
目标/特异性	Detects human Syndecan-1/CD138 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human (rh) Syndecan-2, rhSyndecan-3, rhSyndecan-4, and recombinant mouse Syndecan-1 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard :
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6382 (Human); 20969 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Fibronectin on the Surface of Myeloma Cell-derived Exosomes Mediates Exosome-Cell Interactions. Authors: Purushothaman A, Bandari S, Liu J, Mobley J, Brown E, Sanderson R J Biol Chem, 2016;291(4):1652-63. Species: Human Sample Type: Whole Cells Application: Flow Shed syndecan-1 translocates to the nucleus of cells delivering growth factors and inhibiting histone acetylation: a novel mechanism of tumor-host cross-talk. Authors: Stewart M, Ramani V, Sanderson R J Biol Chem, 2015;290(2):941-9. Species: Human Sample Type: Cell Lysates Application: WB Novel processed form of syndecan-1 shed from SCC-9 cells plays a role in cell migration. Authors: Aragao A, Belloni M, Simabuco F, Zanetti M, Yokoo S, Domingues R, Kawahara R, Pauletti B, Goncalves A, Agostini M, Graner E, Coletta R, Fox J, Leme A PLoS ONE, 2012;7(8):e43521. Species: Human Sample Type: Whole Cells Application: ICC Heparanase Plays a Dual Role in Driving Hepatocyte Growth Factor (HGF) Signaling by Enhancing HGF Expression and Activity. Authors: Ramani VC, Yang Y, Ren Y J. Biol. Chem., 2011;286(8):6490-9. Species: Human Sample Type: Cell Culture Supernates Application: Immunodepletion CD28-mediated regulation of multiple myeloma cell proliferation and survival. Authors: Bahlis NJ, King AM, Kolonias D, Carlson LM, Liu HY, Hussein MA, Terebelo HR, Byrne GE, Levine BL, Boise LH, Lee KP Blood, 2007;109(11):5002-10. Species: Human Sample Type: Whole Tissue Application: IHC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Syndecan-1/CD138 Gln18-Glu251 Accession # NP_002988
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Human Syndecan-1 (also known as CD138) is a variably glycosylated, dimeric, type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 310 amino acid (aa) precursor that contains a 17 aa signal sequence, a 234 aa extracellular domain (ECD), a 25 aa TM segment, and a 34 aa cytoplasmic region. The ECD shows various degrees of heparan sulfate and chondroitin sulfate modification, leading to native molecular weights for Syndecan-1 of 120 - 200 kDa. Proteolytic cleavage of the membrane-bound ECD yields soluble forms of approximately the same molecular weight. Syndecan-1 is an epithelial cell Syndecan involved in Wnt and chemokine signaling. Human Syndecan-1 ECD shares 71% aa identity with the ECD of both rat and mouse Syndecan-1.
运输条件	Blue Ice
存放说明	4℃
参考文献

参考图片

Syndecan‑1/CD138 in Human Ileum. Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human ileum using Human Syndecan‑1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Syndecan‑1/CD138 in Human Jejunum. Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human jejunum using Human Syndecan‑1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.