| 货号 | AF277-SP |
| 别名 | antigen identified by monoclonal R1; CAML1; CAML1N-CAML1; CD171 antigen; CD171; HSAS; HSAS1; L1 cell adhesion molecule; MASA; MIC5; N-CAM-L1; NCAM-L1; neural cell adhesion molecule L1; S10; SPG1 | 全称 | Cell Adhesion Molecule L1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human L1CAM in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) ICAM-2 and rhICAM-3 is observed and less than 1% cross‑reactvity with rhCD31 and recombinant mouse VCAM-1 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3897 (Human); 16728 (Mouse); 50687 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The L1 cell adhesion molecule is a potential biomarker of human distal nephron injury in acute tubular necrosis. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human L1CAM Ile20-Glu1120 Accession # CAA42508 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | L1CAM (Neural cell adhesion molecule L1, also known as L1, CD171 and NCAM-L1) is a 200-230 kDa member of the L1 family, Immunoglobulin (Ig) superfamily of molecules. L1 is recognized to play a key role in cell migration, adhesion, neurite outgrowth, myelination and neuronal differentiation. It does so through a series of cisandtrans interactions that involve multiple copartners and target receptors. L1 is described as forming both homotypic and heterotypic complexes, the latter with molecules as diverse as the EGFR, NCAM, CD24, neurocan and various alpha v plus beta 1and beta 3 integrins. Cells known to express L1 include immature oligodendrocytes, CD4+ T cells, B cells and monocytes, premyelinating Schwann cells, intestinal epithelial progenitor cells, and cerebellar granule plus Purkinje cells. Mature human L1 is a 1238 amino acid (aa) type I transmembrane protein. It contains an 1101 aa extracellular region (aa 20-1120) plus a 114 aa cytoplasmic domain (aa 1144-1257). The extracellular region possesses six C2-type Ig-like domains (aa 35-607) followed by five fibronectin (FN) type III repeats (aa 612-1108). The cytoplasmic tail contains no kinase motifs, but does possess a FIGQY peptide that interacts with ankyrin, and an RSLE sequence that mediates clathrin-associated endocytosis. At least five Ser residues are known to be phosphorylated. There are two splice variants, one each in the intracellular and extracellular domain. A deletion of RSLE (aa 1177-1180) adversely affects endocytosis, while a Leu substitution for aa 26-31 interfers with numerous heterotypic interactions. In general, the full-length L1 molecule is a neuron-associated isoform. L1 is known to undergo proteolysis, either by plasmin or ADAMs. This generates soluble isoforms of varying sizes (140-200 kDa) that retain bioactivity, and which can be incorporated into the surrounding ECM. The membrane fragments (30-80 kDa) undergo further processing, most importantly by gamma ‑secretase, to generate a soluble 28 kDa intracellular domain. This domain is SUMOylated, and believed to possess an NLS at Lys1147. Upon presumed entry into the nucleus, L1 is posited to activate L1-responsive genes. In the extracellular region, human and mouse L1 share 86% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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L1CAM in Human Brain. L1CAM was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human L1CAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF277) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes and cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
