| 货号 | AF2746-SP |
| 别名 | basic helix-loop-helix transcription factor; BETA2; BHF-1; BHLHA3; bHLHa3beta-cell E-box transactivator 2; Class A basic helix-loop-helix protein 3; MODY6; NeuroD; NeuroD1; neurogenic differentiation 1; neurogenic differentiation factor 1; neurogenic helix-loop-helix protein NEUROD; NIDDM | 全称 | Neurogenic Differentiation Factor 1 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects NeuroD1 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human NeuroD2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4760 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Inhibition of medulloblastoma tumorigenesis by the antiproliferative and pro-differentiative gene PC3. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human NeuroD1 Ser154-Asp356 Accession # Q13562 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | NeuroD1, also known as beta-cell E-box transactivator 2 (Beta 2), is abasic helix-loop-helix transcription factor. It is expressed duringdevelopment in various neurons of the central and peripheral nervoussystems. NeuroD1 is also expressed in beta-cells in the pancreas whereit functions both as a transcriptional activator and repressor. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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NeuroD1 in beta TC-6 Mouse Cell Line. Neurogenic Differentiation factor 1 (NeuroD1) was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse NeuroD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2746) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
