Human FGF-9 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human FGF-9 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-273-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-273-SP
别名	FGF9; FGF-9; fibroblast growth factor 9 (glia-activating factor); Fibroblast growth factor 9; GAF; glia-activating factor; HBFG-9; HBGF-9; Heparin-binding growth factor 9; MGC119914; MGC119915; SYNS3
全称	Fibroblast Growth Factor 9
反应种属	Human
应用	Western Blot,Immunohistochemistry
目标/特异性	Detects human FGF-9 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FGF‑4, rhFGF-5, rhFGF-6, rhFGF-7, rhFGF-8, rhFGF-10, rhFGF-17, rhFGF-18, and rhFGF-19 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2254 (Human); 14180 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases. Authors: Yin H, Frontini M, Arpino J, Nong Z, ONeil C, Xu Y, Balint B, Ward A, Chakrabarti S, Ellis C, Gros R, Pickering J J Biol Chem, 2015;290(36):22127-42. Species: Human Sample Type: Tissue Homogenates Application: WB Relationship between the localization of fibroblast growth factor 9 in prostate cancer cells and postoperative recurrence. Authors: Teishima J, Shoji K, Hayashi T Prostate Cancer Prostatic Dis., 2012;15(1):8-14. Species: Human Sample Type: Whole Cells Application: Neut Fibroblast growth factor 9 delivery during angiogenesis produces durable, vasoresponsive microvessels wrapped by smooth muscle cells. Authors: Frontini MJ, Nong Z, Gros R, Drangova M, ONeil C, Rahman MN, Akawi O, Yin H, Ellis CG, Pickering JG Nat. Biotechnol., 2011;29(5):421-7. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF-9 Met1-Ser208 Accession # P31371
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The FGF family is comprised of at least nine polypeptides that show a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. All FGFs have two conserved cysteine residues and share 30-50% sequence identity at the amino acid level. FGF-9, also named glia-activating factor, was originally identified and purified from the supernatant of a human glioma cell line as a heparin-binding mitogenic growth factor for glial cells. FGF-9 has also been shown to stimulate the proliferation of oligodendrocyte type 2 astrocyte progenitor cells, Balb/c3T3 fibroblasts, and PC-12 cells. However, unlike FGF acidic and basic, FGF-9 is not a mitogen for human umbilical vein endothelial cells. The human FGF-9 cDNA encodes a 208 amino acid residue protein that contains a potential N-linked glycosylation site. The native protein is glycosylated. FGF-9 exhibits approximately 30% sequence similarity to other members of the FGF family. Although FGF-9 lacks a typical secretion signal, the protein is secreted efficiently after synthesis. Rat FGF-9 cDNA has been cloned and shown to be highly homologous to human FGF-9. The two proteins differ only in one amino acid residue. The expression of the FGF-9 transcripts has been shown to be restricted to the brain and the kidney.
运输条件	Blue Ice
存放说明	4℃
参考文献	Naruo, K. et al. (1993) J. Biol. Chem. 268:2857. Miyamoto, M. et al. (1993) Mol. Cell Biol. 13:4251.

参考图片

FGF‑9 in Human Placenta. FGF‑9 was detected in immersion fixed paraffin-embedded sections of human placenta using 5 µg/mL Goat Anti-Human FGF‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑273‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.