Human CD27 Ligand/TNFSF7 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human CD27 Ligand/TNFSF7 Affinity Purified Polyclonal Ab (25 UG)

货号： AF2738-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2738-SP
别名	CD27-L; CD27LCD27 ligand; CD27LG; CD70 molecule; CD70; Ki-24 antigen; surface antigen CD70; TNFSF7; TNFSF7CD70 antigen; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7
反应种属	Human
应用	Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human CD27 Ligand in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse CD27 Ligand and recombinant human (rh) CD30 Ligand is observed, and less than 1% cross-reactivity with rhCD40 Ligand is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	970 (Human); 21948 (Mouse)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human CD27 Ligand/TNFSF7 Gln45-Pro193 Accession # AAA36175
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Human CD27 Ligand (also CD70) is a 30 kDa type 2 transmembrane glycoprotein that is a member of the TNF superfamily. It contains a 20 amino acid (aa) cytoplasmic region and a 155 aa extracellular domain that shows multiple beta -strands. It is an inducible trimer that occurs on B cells, T cells, and NK cells. It regulates T cell dependant B cell differentiation into plasma cells and likely to promotes clonal expansion of T cells. Human CD27 Ligand extracellular region is 64% aa identical to mouse and rat CD27 Ligand extracellular regions.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Development and Differentiation Co-stimulatory and Co-inhibitory Molecules gamma delta T Cells Human CD Marker Products Regulation of Apoptosis by TNF Superfamily Members Regulation of B Cell Activation and Homeostasis by TNF Superfamily Members Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members Regulation of T Cell Co-stimulation by TNF Superfamily Members Regulatory T Cells (Tregs) TNF Superfamily TNF Superfamily Ligands

参考图片

Detection of CD27 Ligand/TNFSF7 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitts lymphoma cell line was stained with Human CD27 Ligand/TNFSF7 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2738, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Detection of Human CD27 Ligand/TNFSF7 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human CD27 Ligand/TNFSF7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2738) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD27 Ligand/TNFSF7 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.