Human CCL4/MIP-1 beta Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human CCL4/MIP-1 beta Affinity Purified Polyclonal Ab (25 UG)

货号： AF-271-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-271-SP
别名	ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2
反应种属	Human
应用	Western Blot,Immunohistochemistry,Immunocytochemistry,Neutralization
目标/特异性	Detects human CCL4/MIP-1 beta in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) MIP-1 alpha is observed, approximately 20% cross-reactivity with recombinant human (rh) MIP-1 alpha and recombinant cotton rat MIP-1 alpha is observed, and less than 1% cross-reactivity with rhMCP-2, rmMIP-1 beta, rmMCP-2, and recombinant viral MIP-1 beta is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize CCL4/MIP‑1 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically 0.3-1.5 µg/mL in the presence of 40 ng/mL Recombinant Human CCL4/MIP‑1 beta.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Increased Levels of Macrophage Inflammatory Proteins Result in Resistance to R5-Tropic HIV-1 in a Subset of Elite Controllers. Authors: Walker W, Kurscheid S, Joshi S, Lopez C, Goh G, Choi M, Barakat L, Francis J, Fisher A, Kozal M, Zapata H, Shaw A, Lifton R, Sutton R, Fikrig E J Virol, 2015;89(10):5502-14. Species: Human Sample Type: Whole Cells Application: Neut NK cell responses to simian immunodeficiency virus vaginal exposure in naive and vaccinated rhesus macaques. Authors: Shang L, Smith A, Duan L, Perkey K, Qu L, Wietgrefe S, Zupancic M, Southern P, Masek-Hammerman K, Reeves R, Johnson R, Haase A J Immunol, 2014;193(1):277-84. Species: Primate - Macaca mulatta (Rhesus Macaque) Sample Type: Whole Tissue Application: IHC A novel p38-MAPK signaling axis modulates neutrophil biology in head and neck cancer. Authors: Dumitru CA, Fechner MK, Hoffmann TK J. Leukoc. Biol., 2012;91(4):591-8. Species: Human Sample Type: Whole Cells Application: ICC Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease. Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS J. Immunol., 2009;182(6):3919-27. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Intestinal neuro-epithelial interactions modulate neuronal chemokines production. Authors: Tixier E, Galmiche JP, Neunlist M Biochem. Biophys. Res. Commun., 2006;344(2):554-61. Species: Human Sample Type: Whole Cells Application: ICC The beta chemokines CCL4 and CCL5 enhance adhesion of specific CD4+ T cell subsets to human brain endothelial cells. Authors: Quandt J, Dorovini-Zis K J. Neuropathol. Exp. Neurol., 2004;63(4):350-62. Species: Human Sample Type: Whole Cells Application: ICC Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes. Authors: Binninger-Schinzel D, Norley S, Adler HS, Oberg HH, Kurth R Virology, 2002;294(1):209-21. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CCL4/MIP-1 beta is a beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. It attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. It signals through CCR5, and an N-terminally trimmed form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor.
运输条件	Blue Ice
存放说明	4℃
参考文献	CC Chemokines and Receptors Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis Infectious Diseases Inflammatory Mediators Macrophage Activation Markers Molecules Secreted by VSMC Neuroinflammation Regulatory T Cells (Tregs)

参考图片

CCL4/MIP‑1 beta in Human Alzheimers Disease Brain.

CCL4/MIP‑1 beta was detected in immersion fixed paraffin-embedded sections of human Alzheimers disease brain (hippocampus) using 15 µg/mL Goat Anti-Human CCL4/MIP‑1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑271‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Chemotaxis Induced by CCL4/MIP‑1 beta and Neutralization by Human CCL4/MIP‑1 beta Antibody.

Recombinant Human CCL4/MIP‑1 beta (Catalog # 271-BME) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL4/MIP‑1 beta (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL4/MIP‑1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-271-NA). The ND₅₀ is typically 0.3-1.5 µg/mL.

CCL4/MIP‑1 beta in Human PBMCs.

CCL4/MIP‑1 beta was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA and monensin using Goat Anti-Human CCL4/MIP‑1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-271-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.