Human IL-1 RI Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-1 RI Affinity Purified Polyclonal Ab (25 UG)

货号： AF269-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF269-SP
别名	CD121 antigen-like family member A; CD121a antigen; CD121a; IL1R; IL1R1; IL1RT1; IL-1RT1; IL-1RT-1; Interleukin 1 Receptor 1; interleukin 1 receptor, type I; interleukin receptor 1; interleukin-1 receptor type 1; p80
全称	Interleukin 1 Receptor I
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects human IL-1 RI in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Neutralization: Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced growth inhibition in the A375s2 human melanoma cell line. The Neutralization Dose (ND₅₀) is typically 0.5-2.0 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL‑1 beta /IL‑1F2.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3554 (Human); 16177 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Expression and function of IL-1R8 (TIR8/SIGIRR), a regulatory member of the IL-1 receptor family in platelets Authors: Achille Anselmo Cardiovasc Res, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Flow Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways. Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D J Immunol, 2015;195(6):2818-28. Species: Human Sample Type: Whole Cells Application: ICC Fluorescence Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma. Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R PLoS ONE, 2015;10(1):e0116772. Species: Human Sample Type: Whole Cells Application: Blocking Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F Nature, 2012;484(7395):514-8. Species: Human Sample Type: Whole Cells Application: Neut Chorionic gonadotropin down-regulates the expression of the decoy inhibitory interleukin 1 receptor type II in human endometrial epithelial cells. Authors: Herrmann-Lavoie C, Rao CV, Akoum A Endocrinology, 2007;148(11):5377-84. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians. Authors: Jantra S, Bigliardi E, Brizzi R, Ietta F, Bechi N, Paulesu L Biol. Reprod., 2007;76(6):1009-15. Species: Amphibian - B. bufo (common toad) Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-1 RI
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex. An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.
运输条件	Blue Ice
存放说明	4℃
参考文献	Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

参考图片

Detection of IL‑1 RI in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were labeled with Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Growth Inhibition Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 RI Antibody. Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) inhibits proliferation in the A375s2 human melanoma cell line in a dose-dependent manner (orange line). Growth inhibition elicited by Recombinant Human IL‑1 beta /IL‑1F2 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269). The ND₅₀ is typically 0.5-2.0 µg/mL.