货号 | AF2697-SP |
别名 | DNA-binding factor KBF1; EBP-1; NF-kappa-B; NFKB1; NF-kB1; NFKB-p105; Nuclear factor NF-kappa-B p105 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; p105 | 全称 | Nuclear Factor Kappa-B, Subunit 1 |
反应种属 | Human/Mouse |
应用 | Western Blot(0.5 µg/mL) Chromatin Immunoprecipitation (ChIP)(5 µg/5 x 106cells) |
目标/特异性 | Detects endogenous human and mouse NF kappa B1 in Western blots. |
使用方法 | Western Blot: 0.5 µg/mL Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 106cells |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 4790 (Human); 18033 (Mouse) |
纯化方式 | Antigen Affinity-purified |
免疫原 | E. coli-derived recombinant human NF kappa B1 Pro317-Asp437 Accession # NP_003989 |
生物活性 | Human, Mouse |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | Nuclear Factor kappa B1 (NF kappa B1 or NF kappa B p50) is a member of the rel/NF kappa B family of transcription factors. NF kappa B1 dimerizes with other members of the rel/NF kappa B family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Human NF kappa B1 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse NF kappa B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2697) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for NF kappa B1 at approximately 50 and 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of NF kappa B1-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. NF kappa B1/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse NF kappa B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2697) or control antibody (Catalog # AB‑108‑C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The bcl-2 promoter was detected by standard PCR. This application has not been tested in mouse samples. |