Human/Mouse NFkB1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse NFkB1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF2697-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2697-SP
别名	DNA-binding factor KBF1; EBP-1; NF-kappa-B; NFKB1; NF-kB1; NFKB-p105; Nuclear factor NF-kappa-B p105 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; p105
全称	Nuclear Factor Kappa-B, Subunit 1
反应种属	Human/Mouse
应用	Western Blot(0.5 µg/mL) Chromatin Immunoprecipitation (ChIP)(5 µg/5 x 10⁶cells)
目标/特异性	Detects endogenous human and mouse NF kappa B1 in Western blots.
使用方法	Western Blot: 0.5 µg/mL Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4790 (Human); 18033 (Mouse)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human NF kappa B1 Pro317-Asp437 Accession # NP_003989
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Nuclear Factor kappa B1 (NF kappa B1 or NF kappa B p50) is a member of the rel/NF kappa B family of transcription factors. NF kappa B1 dimerizes with other members of the rel/NF kappa B family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes.
运输条件	Blue Ice
存放说明	4℃
参考文献	Apoptosis Transcription Factors and Regulators Cancer Stem Cell Transcription Factors Chemokine Receptor Intracellular Signaling C-type Lectin Receptors Diabetic Peripheral Neuropathy EMT Induction IL-1 Family IL-17 Family Immunoglobulin-Like Domain Transcription Factors Intracellular Signaling Molecules in Angiogenesis Myeloid-derived Suppressor Cells (MDSC) Natural Killer T (NKT) Cells Neurotrophin/Trk Family NFkB Pathway NOD-like Receptors and the Inflammasome Notch Pathway PDGF Family Pluripotent Stem Cell Transcription Factors Positive Regulators of the Jak/STAT Pathway RIG-I-like Receptors TNF Superfamily Regulators Toll-Like Receptors Transcription Factors and Regulators Transcription Factors in the Akt Pathway Transcriptional Regulators in Allergy and Asthma

参考图片

Detection of Human NF kappa B1 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse NF kappa B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2697) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for NF kappa B1 at approximately 50 and 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of NF kappa B1-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. NF kappa B1/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse NF kappa B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2697) or control antibody (Catalog # AB‑108‑C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The bcl-2 promoter was detected by standard PCR. This application has not been tested in mouse samples.