| 货号 | AF2684-SP |
| 别名 | FIP-3IP2; FIP3NF-kappa-B essential modifier; FIP3P; I-kappa-B kinase subunit gamma; IkB kinase gamma subunit; IkB kinase subunit gamma; IkB kinase-associated protein 1; IKBKG; IKKAP1; IKKG; IKK-gammaIPD2; incontinentia pigmenti; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma; Inhibitor of nuclear factor kappa-B kinase subunit gamma; IP; IP1; NEMO; NEMOAMCBX1; NFkappaB essential modulator; NF-kappa-B essential modulator | 全称 | IkB Kinase gamma |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat IKK gamma in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8517 (Human); 309295 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human IKK gamma Met1-Glu419 Accession # Q9Y6K9 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | I kappa B kinase gamma (IKK gamma ) is also known as NF-kappa B essential modulator (NEMO), IKBKG, and FIP3. The active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma (50 and 55 kDa), phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. The regulatory IKK gamma is required for activation of the IKK complex. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat IKK gamma by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2684) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human IKK gamma (1 ng) was included. Specific bands for IKK gamma were detected at approximately 50 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human IKK gamma by Simple WesternTM. Simple Western lane view shows lysates of MDA‑MB‑468 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for IKK gamma at approximately 47-56 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2684) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. | |
IKK gamma in MCF‑7 Human Cell Line. IKK gamma was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2684) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
