货号 | AF2679-SP |
别名 | B lymphocyte kinase; B lymphoid tyrosine kinase; BLK nonreceptor tyrosine kinase; EC 2.7.10.2; MGC10442; MODY11; p55-Blk; tyrosine-protein kinase Blk | 全称 | B Lymphoid Tyrosine Kinase |
反应种属 | Human/Mouse/Rat |
应用 | Western Blot(0.5 µg/mL) |
目标/特异性 | Detects human, mouse, and rat Blk in Western blots. |
使用方法 | Western Blot: 0.5 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 640 (Human); 12124 (Mouse); 364403 (Rat) |
纯化方式 | Antigen Affinity-purified |
免疫原 | E. coli-derived recombinant human Blk Met1-Val63 Accession # P51451 |
生物活性 | Human, Mouse, Rat |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | B-cell lymphocyte kinase (Blk) is a member of the Src family of non‑receptor tyrosine kinases. Constitutively active Blk induces malignant transformation of B-cell progenitors, suggesting a role for Blk in B-lineage cell differentiation and proliferation. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Human/Mouse/Rat Blk by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Blk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2679) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Blk at approximately 55 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |