货号 | AF2670-SP |
别名 | Activator protein 1; AP1; AP-1; c-Jun; enhancer-binding protein AP1; Jun activation domain binding protein; jun oncogene; jun proto-oncogene; JUN; Proto-oncogene c-Jun; transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homologp39; v-jun sarcoma virus 17 oncogene homolog (avian); v-jun sarcoma virus 17 oncogene homolog | 全称 | Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1 |
反应种属 | Human |
应用 | Western Blot(0.5 µg/mL) |
目标/特异性 | Detects human c-Jun in Western blots. |
使用方法 | Western Blot: 0.5 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 3725 (Human) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Characterization of short range DNA looping in endotoxin-mediated transcription of the murine inducible nitric-oxide synthase (iNOS) gene. | |
纯化方式 | Antigen Affinity-purified |
免疫原 | E. coli-derived recombinant human c-Jun Thr2-Phe331 Accession # P05412 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | c-Jun is one of three Jun family proteins making up the Activator Protein-1 (AP-1) transcription factor group. c-Jun acts as a positive regulator of cell proliferation and can induce oncogenic transformation. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Human c-Jun by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MDA‑MB‑468 human breast cancer cell lines. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human c-Jun Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2670) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for c-Jun at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |