| 货号 | AF2668-SP |
| 别名 | EC 3.1.3.48; MEG2; protein tyrosine phosphatase, non-receptor type 9; Protein-tyrosine phosphatase MEG2; PTPase MEG2; PTPase-MEG2; PTPMEG2; PTPN9; tyrosine-protein phosphatase non-receptor type 9 | 全称 | Megakaryocyte Protein Tyrosine Phosphatase 2 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat PTP-MEG2 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5780 (Human); 56294 (Mouse); 266611 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PTP-MEG2 Met1-Gln593 Accession # P43378 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Megakaryocyte protein tyrosine phosphatase 2 (PTP-MEG2), also known as PTPN9, contains an N-terminal domain homologous to the yeast retinaldehyde binding domain Sec14p and a C-terminal phosphatase domain. The Sec14p domain targets PTP-MEG2 to the membrane of secretory vesicles, where it promotes vesicle fusion. Elevated levels of membrane bound PTP-MEG2 activity in erythroid progenitor cells are observed in polycythemia vera and may affect responsiveness to colony stimulating factors. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human, Mouse, and Rat PTP‑MEG2 by Western Blot. Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line, CTLL‑2 mouse cytotoxic T cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PTP‑MEG2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2668) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PTP‑MEG2 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
