| 货号 | AF2660-SP |
| 别名 | DKFZp586P2421; KSPG25; mimecan proteoglycan; mimecan; OGN; OIF; OIFOG; osteoglycin (osteoinductive factor); osteoglycin; Osteoinductive factor; SLRR3A; SLRR3Acorneal keratan sulfate proteoglycan |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects human Mimecan in direct ELISAs and Western blots. In these formats, approximately 5% cross‑reactivity with recombinant mouse Mimecan is observed. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4969 (Human); 18295 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Mimecan Ala20-Phe298 Accession # P20774 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mimecan, also known as KSPG25 (25 kDa Keratan Sulfate Proteoglycan) and Osteoglycin, is an extracellular matrix protein belonging to the class III subfamily of the small leucine rich proteoglycan (SLRP) family. SLRPs are characterized by leucine‑rich repeats in the central domain, which is flanked by N- and C-terminal cysteine-rich domains. The core proteins contain N-linked oligosaccharides as well as glycosaminoglycan side chains. Mimecan is synthesized as a preproprotein that is processed to generate proteins of different sizes including Osteoglycin (containing 93 most C-terminal amino acid residues) and Mimecan (containing 223 most C-terminal residues). Human Mimecan shares approximately 85% amino acid sequence homology with the mouse or rat Mimecan. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Mimecan by Western Blot. Western blot shows lysates of human aorta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mimecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2660) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Mimecan at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human Mimecan by Simple WesternTM. Simple Western lane view shows lysates of human heart (aorta) tissue, loaded at 0.2 mg/mL. A specific band was detected for Mimecan at approximately 44 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Mimecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2660) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
