| 货号 | AF265-SP |
| 别名 | ALS9; ANG; angiogenin; angiogenin, ribonuclease, RNase A family, 5; EC 3.1.27; EC 3.1.27.-; epididymis luminal protein 168; HEL168; MGC22466; MGC71966; Ribonuclease 5; RNase 5; RNASE5RNASE4 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Angiogenin in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 283 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Angiogenin Gln25-Pro147 Accession # Q53X86 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Angiogenin was initially purified from serum-free media conditioned by growth of a human adenocarcinoma cell line HT-29 based on its ability to initiate vascularization in the chicken embryo chorioallantoic membrane. A number of other tumor, as well as normal, cell lines can also secrete Angiogenin. In addition, Angiogenin is present in normal human plasma at levels as high as 60‑120 ng/mL. Unlike other angiogenic factors such as FGF, Angiogenin is neither mitogenic nor chemotactic for vascular endothelial cells in vitro. However, Angiogenin can stimulate capillary and umbilical vein endothelial cells to produce diacylglycerol and secrete prostacyclin by phospholipase activation. Angiogenin, absorbed on plastic, can also support endothelial and fibroblast cell adhesion and spreading. Surprisingly, Angiogenin has been found to be a member of the ribonuclease superfamily with approximately 35% sequence similarity at the amino acid level with pancreatic RNase. Angiogenin exhibits ribonucleolytic activity that is distinctly different than that of pancreatic RNase A. The ribonucleolytic activity of Angiogenin toward most RNase A substrates is much lower than that of RNase A. Nevertheless, the ribonucleolytic activity of Angiogenin is essential to its angiogenic activity since inhibition of the Angiogenin RNase activity will also abolish angiogenesis activity. Similar to several members of the RNase superfamily, Angiogenin is a cytotoxic agent that can abolish cellular protein synthesis. It has been demonstrated that Angiogenin-dependent protein synthesis inhibition can be attributed to the function of Angiogenin as a cytotoxic tRNA-specific RNAase. A cell-surface Angiogenin binding protein has been purified and characterized. Tryptic peptide mapping and sequence analysis indicate that this binding protein is a member of the actin family. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human Angiogenin by Western Blot. Western blot shows lysates of human epithelium and placenta tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Angiogenin at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
