Human Nectin-4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Nectin-4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF2659-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2659-SP
别名	EDSS1; Ig superfamily receptor LNIR; LNIR; LNIRnectin 4; nectin-4; poliovirus receptor-related 4; poliovirus receptor-related protein 4; PRR4; PRR4DKFZp686K05193; PVRL4
全称	Poliovirus Receptor Related 4
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross‑reactivity with recombinant mouse Nectin-4 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	81607 (Human); 71740 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Nectin-4 expression contributes to tumor proliferation, angiogenesis and patient prognosis in human pancreatic cancer. Authors: Nishiwada S, Sho M, Yasuda S, Shimada K, Yamato I, Akahori T, Kinoshita S, Nagai M, Konishi N, Nakajima Y J Exp Clin Cancer Res, 2015;34(0):30. Species: Human Sample Type: Whole Tissue Application: IHC - paraffin SLAM- and Nectin-4-Independent Noncytolytic Spread of Canine Distemper Virus in Astrocytes. Authors: Alves L, Khosravi M, Avila M, Ader-Ebert N, Bringolf F, Zurbriggen A, Vandevelde M, Plattet P J Virol, 2015;89(10):5724-33. Species: Canine Sample Type: tissue Application: IHC Oncolytic measles virus expressing the sodium iodide symporter to treat drug-resistant ovarian cancer. Authors: Galanis E, Atherton P, Maurer M, Knutson K, Dowdy S, Cliby W, Haluska P, Long H, Oberg A, Aderca I, Block M, Bakkum-Gamez J, Federspiel M, Russell S, Kalli K, Keeney G, Peng K, Hartmann L Cancer Res, 2015;75(1):22-30. Species: Human Sample Type: Whole Tissue Application: IHC - Not specified Lethal canine distemper virus outbreak in cynomolgus monkeys in Japan in 2008. J Virol, 2013;87(2):1105-14. Species: Primate - Macaca fascicularis (Crab-eating or Cynomolgus macaque) Sample Type: tissue Application: IHC Paraffin-embedded Tumor cell marker PVRL4 (nectin 4) is an epithelial cell receptor for measles virus. Authors: Noyce RS, Bondre DG, Ha MN PLoS Pathog., 2011;7(8):e1002240. Species: Hamster Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Nectin-4 Gly27-Val351 Accession # Q96NY8
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectinfamily of Ig superfamily proteins. It is both a homophilic andheterophilic (with Nectin-1) cell adhesion molecule that is expressedin the embryo and in breast carcinoma. A soluble form of Nectin-4 isgenerated from the membrane protein via the action of TACE/ADAM-17. Theextracellular domain of human Nectin-4 shares 90% and 92% amino acidsequence homology with the corresponding regions of mouse and ratNectin-4, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cell to Cell Adhesion Disassembly During EMT Epithelial Cell Markers and Intracellular Molecules Epithelial Cell Polarity ICC / IHC Images: Nectin-4 Infectious Diseases Nectin and Nectin-like Binding Receptors and Ligands Nectins Surface Ectodermal Cells

参考图片

Nectin‑4 in Human Placenta. Nectin‑4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 µg/mL Goat Anti-Human Nectin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2659) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.