| 货号 | AF2658-SP |
| 别名 | MGC11078; TNFRSF1A-associated via death domainTNFR1-associated DEATH domain protein; tumor necrosis factor receptor type 1 associated death domain protein; tumor necrosis factor receptor type 1-associated DEATH domain protein; tumor necrosis factor receptor-1-associated protein | 全称 | TNFRSF1A-associated via Death Domain |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human TRADD. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8717 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TRADD Met1-Ala312 Accession # Q15628 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TNF RI (Tumor Necrosis Factor Receptor I) activation leads to the recruitment of the adapter protein TRADD (TNF RI-associated Death Domain) to the cytoplasmic death domain of TNF RI. The TRADD protein also contains a death domain with sequence similarity to that of TNF RI. In addition to the initiation of signaling events leading to apoptosis, the recruitment of TRADD or over-expression of the TRADD protein also activates the transcription factor NFkB. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TRADD by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line, MCF‑7 human breast cancer cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRADD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2658) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRADD at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4. |
