Human PlGF Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human PlGF Affinity Purified Polyclonal Ab (25 UG)

货号： AF-264-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-264-SP
别名	D12S1900; PGF; PGFL; placenta growth factor; placental growth factor; placental growth factor, vascular endothelial growth factor-related protein; PlGF; PlGF-2; PLGFplacental growth factor-like; SHGC-10760
全称	Placenta Growth Factor
反应种属	Human
应用	Western Blot
目标/特异性	Detects human PlGF (but not VEGF) in direct ELISAs and Western blots. This antibody will also recognize the heterodimer of VEGF and PlGF. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse PlGF-2 is observed.
使用方法	Western Blot: 2 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5228 (Human); 18654 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model. Authors: Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST Angiogenesis, 2010;13(4):337-47. Species: Human Sample Type: Whole Cells Application: Neut VEGF-A and VEGF-F evoke distinct changes in vascular ultrastructure. Authors: Matsunaga Y, Yamazaki Y, Suzuki H, Morita T Biochem. Biophys. Res. Commun., 2009;379(4):872-5. Species: Guinea Pig Sample Type: Whole Tissue Application: IHC Malignant mesothelioma growth inhibition by agents that target the VEGF and VEGF-C autocrine loops. Authors: Masood R, Kundra A, Zhu S, Xia G, Scalia P, Smith DL, Gill PS Int. J. Cancer, 2003;104(5):603-10. Species: Human Sample Type: Cell Lysates Application: WB Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome. Authors: Zhou Y, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ Am. J. Pathol., 2002;160(4):1405-23. Species: Human Sample Type: Whole Tissue Application: IHC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human PlGF Ala21-Arg149 Accession # CAA38698
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Placenta Growth Factor (PlGF) is a member of the Vascular Endothelial Growth Factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. In their PDGF-like regions, VEGF and PlGF also share approximately 53% amino acid sequence similarity. The gene for PlGF has been mapped to chromosome 14. As a result of alternative splicing, at least two PlGF mRNAs encoding monomeric PlGF precursors containing 149 and 170 amino acid residues have been described. The expression of PlGF is not widespread, but has been detected in human umbilical vein endothelial cells, placenta, choriocarcinoma cell lines, and in renal cell carcinoma associated with angiogenesis. The PlGF proteins bind with high-affinity to Flt-1, but not to Flk-1/KDR.
运输条件	Blue Ice
存放说明	4℃
参考文献	Epithelial Cell Markers and Intracellular Molecules HIF Transcription Factors Preeclampsia Surface Ectodermal Cells VEGF Family VEGF Family Ligands

参考图片

Detection of Human PlGF by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with full length human PlGF. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PlGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-264-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PlGF at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.