| 货号 | AF2647-SP |
| 别名 | HWNT11; MGC141946; MGC141948; protein Wnt-11; wingless-type MMTV integration site family, member 11 | 全称 | Wingless-type MMTV Integration Site Family, Member 11 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Wnt-11 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse (rm) Wnt-3A is observed and less than 1% cross-reactivity with rmWnt-5A is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7481 (Human); 22411 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The noncanonical WNT pathway is operative in idiopathic pulmonary arterial hypertension. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Wnt‑11 peptide Leu39-Ala79 and Lys225-Arg297 Accession # Q059Y4 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Wnt-11 belongs to the Wnt family of secreted, highly conserved, cellsignaling molecules that play important roles in vertebratedevelopment. Mouse Wnt-11 shares 97% amino acid sequence identity withits human and rat homolog. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Wnt‑11 in LNCaP Human Cell Line. Wnt‑11 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Mouse Wnt‑11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2647) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
