| 货号 | AF2637-SP |
| 别名 | FLJ30977; FLJ31092; layilin; LAYN; LYAN |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human Layilin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse Layilin is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 143903 (Human); 244864 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Layilin Ala22-Glu220 Accession # NP_849156 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Layilin (named after the L-A-Y-I-L-I six amino acid motif in the molecule’s transmembrane segment) is a 55 kDa member of the animal C-type lectin family. The human Layilin cDNA encodes a 374 amino acid (aa) type I transmembrane protein that, based on the orthologous molecule in hamster, contains a 19 aa signal sequence, a 201 aa extracellular region, a 31 aa transmembrane segment, and a 123 aa cytoplasmic tail (1, 2). The extracellular domain has a 130 aa carbohydrate recognition domain that contains only one potential Ca++-binding site. The intracellular region contains three types of LH (Layilin homology) repeats. There are three 16‑18 aa LH1 repeats, 3 five aa (E-G-S-F/W-V) LH2 repeats, and 2 four aa (N-D/E-I-Y) LH3 repeats. Adjacent LH2-LH3 tandem arrays provide docking sites for talin, a cytoskeleton-membrane adaptor protein (3). The human Layilin extracellular region is 85% aa identical to the hamster Layilin extracellular region, and 82% aa identical to the extracellular region of a similar molecule identified in mouse. Layilin is known to be a receptor for hyaluronan (HA), a negatively charged linear high molecular weight polysaccharide composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine (1, 4, 5). HA is found in the extracellular matrix of most animal tissues and in body fluids. Thus, it may be that Layilin modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease. Layilin binds specifically to HA but not to other glycosaminoglycans (4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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