| 货号 | AF2606-SP |
| 别名 | GATA binding protein 4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; transcription factor GATA-4 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Chromatin Immunoprecipitation (ChIP)(5 µg/5 x 106cells) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human GATA-4 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 106cells Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2626 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A thyroid hormone receptor/KLF9 axis in human hepatocytes and pluripotent stem cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human GATA-4 Met27-Phe211 Accession # P43694 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | GATA-4 belongs to the GATA family of transcription factors, which bindto the consensus DNA sequence (A/T) GATA (A/G) to control diversetissue-specific programs of gene expression and morphogenesis. It iswidely expressed in mesodermal- and endodermal-derived tissues. GATA-4interacts with other transcription factors such as NKX2.5, and myocyteenhancer factor 2 to regulate cardiac myocyte-specific gene expression.Human GATA-4 share 93%, 93% and 92% amino acid sequence identity withporcine, rat and mouse GATA-4, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human GATA‑4 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GATA‑4 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of GATA‑4-regulated Genes by Chromatin Immunoprecipitation. KATO‑III human gastric carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA‑4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human GATA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The mucin4 promoter was detected by standard PCR. | |
GATA‑4 in Differentiated Human Embryonic Stem Cells. GATA‑4 was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human GATA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
GATA‑4 in Differentiated Human Embryonic Stem Cells. GATA‑4 was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human GATA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
