Human HB-EGF Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human HB-EGF Affinity Purified Polyclonal Ab (25 UG)

货号： AF-259-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-259-SP
别名	diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor); Dtr; DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor); Dts; DTSF; HBEGF; HB-EGF; Hegfl; heparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; proheparin-binding EGF-like growth factor
全称	Heparin Binding EGF-like Growth Factor
反应种属	Human
应用	Western Blot,Immunohistochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human HB-EGF in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) Amphiregulin, rhBetacellulin, rhEpiregulin, and recombinant mouse Epigen is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize HB‑EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. Rubin, J.S. et al. (1991) PNAS 88:415. The Neutralization Dose (ND₅₀) is typically 0.1-0.6 µg/mL in the presence of 20 ng/mL Recombinant Human HB‑EGF.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1839 (Human); 15200 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Osmotic Induction of Angiogenic Growth Factor Expression in Human Retinal Pigment Epithelial Cells. Authors: Veltmann M, Hollborn M, Reichenbach A, Wiedemann P, Kohen L, Bringmann A PLoS ONE, 2016;11(1):e0147312. Species: Human Sample Type: Whole Cells Application: Neut M2 macrophages induce ovarian cancer cell proliferation via a heparin binding epidermal growth factor/matrix metalloproteinase 9 intercellular feedback loop Authors: MJ Carroll, A Kapur, M Felder, MS Patankar, PK Kreeger Oncotarget, 2016;7(52):86608-86620. Species: Human Sample Type: Whole Cells Application: Neut Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow. Authors: Yu V, Saez B, Cook C, Lotinun S, Pardo-Saganta A, Wang Y, Lymperi S, Ferraro F, Raaijmakers M, Wu J, Zhou L, Rajagopal J, Kronenberg H, Baron R, Scadden D J Exp Med, 2015;212(5):759-74. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded MicroRNA-132 enhances transition from inflammation to proliferation during wound healing. Authors: Li D, Wang A, Liu X, Meisgen F, Grunler J, Botusan I, Narayanan S, Erikci E, Li X, Blomqvist L, DU L, Pivarcsi A, Sonkoly E, Chowdhury K, Catrina S, Stahle M, Landen N J Clin Invest, 2015;125(8):3008-26. Species: Human Sample Type: Cell Lysates Application: WB Heparin-binding epidermal growth factor-like growth factor eliminates constraints on activated Kras to promote rapid onset of pancreatic neoplasia. Authors: Ray, K C, Moss, M E, Franklin, J L, Weaver, C J, Higginbotham, J, Song, Y, Revetta, F L, Blaine, S A, Bridges, L R, Guess, K E, Coffey, R J, Crawford, H C, Washington, M K, Means, A L Oncogene, 2014;33(7):823-31. Species: Human Sample Type: Whole Tissue Application: IHC A mouse model of otitis media identifies HB-EGF as a mediator of inflammation-induced mucosal proliferation. Authors: Suzukawa, Keigo, Tomlin, Julia, Pak, Kwang, Chavez, Eduardo, Kurabi, Arwa, Baird, Andrew, Wasserman, Stephen, Ryan, Allen F PLoS ONE, 2014;9(7):e102739. Species: Rat Sample Type: Tissue Homogenates Application: WB An EGFR wild type-EGFRvIII-HB-EGF feed-forward loop regulates the activation of EGFRvIII. Authors: Li L, Chakraborty S, Yang C, Hatanpaa K, Cipher D, Puliyappadamba V, Rehman A, Jiwani A, Mickey B, Madden C, Raisanen J, Burma S, Saha D, Wang Z, Pingle S, Kesari S, Boothman D, Habib A Oncogene, 2014;33(33):4253-64. Species: Human Sample Type: Whole Cells Application: Neut Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions. Authors: Smith A, Bowers S, Stratman A, Davis G PLoS ONE, 2013;8(12):e85147. Species: Human Sample Type: Whole Cells Application: Neut Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia. Authors: Diaz B, Yuen A, Iizuka S, Higashiyama S, Courtneidge S J Cell Biol, 2013;201(2):279-92. Immature doublecortin-positive hippocampal neurons are important for learning but not for remembering. Authors: Vukovic, Jana, Borlikova, Gilyana, Ruitenberg, Marc J, Robinson, Gregory, Sullivan, Robert K, Walker, Tara L, Bartlett, Perry F J Neurosci, 2013;33(15):6603-13. Species: Mouse Sample Type: Whole Tissue Application: IHC - Paraffin embedded Amphiregulin carboxy-terminal domain is required for autocrine keratinocyte growth. Authors: Stoll SW, Johnson JL, Li Y, Rittie L, Elder JT J. Invest. Dermatol., 2010;130(8):2031-40. Species: Human Sample Type: Whole Cells Application: Neut Heparin binding epidermal growth factor in renal ischaemia/reperfusion injury. Authors: Mulder GM, Nijboer WN, Seelen MA, Sandovici M, Bos EM, Melenhorst WB, Trzpis M, Kloosterhuis NJ, Visser L, Henning RH, Leuvenink HG, Ploeg RJ, Sunnarborg SW, van Goor H J. Pathol., 2010;221(2):183-92. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded HER family receptor abnormalities in lung cancer brain metastases and corresponding primary tumors. Authors: Sun M, Behrens C, Feng L, Ozburn N, Tang X, Yin G, Komaki R, Varella-Garcia M, Hong WK, Aldape KD, Wistuba II Clin. Cancer Res., 2009;15(15):4829-37. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Wounding-induced synthesis of hyaluronic acid in organotypic epidermal cultures requires the release of heparin-binding egf and activation of the EGFR. Authors: Monslow J, Sato N, Mack JA, Maytin EV J. Invest. Dermatol., 2009;129(8):2046-58. Species: Human Sample Type: Recombinant Protein Application: WB EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells. Authors: Liu M, Yang SC, Sharma S, Luo J, Cui X, Peebles KA, Huang M, Sato M, Ramirez RD, Shay JW, Minna JD, Dubinett SM Am. J. Respir. Cell Mol. Biol., 2007;37(5):578-88. Species: Human Sample Type: Whole Cells Application: Neut Expression and role of EGFR ligands induced in airway cells by PM2.5 and its components. Authors: Rumelhard M, Ramgolam K, Hamel R, Marano F, Baeza-Squiban A Eur. Respir. J., 2007;30(6):1064-73. Species: Human Sample Type: Whole Cells Application: Neut Ras activation in response to phorbol ester proceeds independently of the EGFR via an unconventional nucleotide-exchange factor system in COS-7 cells. Authors: Rubio I, Rennert K, Wittig U, Beer K, Durst M, Stang SL, Stone J, Wetzker R Biochem. J., 2006;398(2):243-56. Species: Primate - Chlorocebus pygerythrus (Vervet Monkey) Sample Type: Cell Lysates Application: WB Role of epidermal growth factor receptor transactivation in alpha1B-adrenoceptor phosphorylation. Authors: Casas-Gonzalez P, Garcia-Sainz JA Eur. J. Pharmacol., 2006;542(1):31-6. Species: Rat Sample Type: Whole Cells Application: Neut Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration. Authors: Li Y, Fan J, Chen M, Li W, Woodley DT J. Invest. Dermatol., 2006;126(9):2096-105. Species: Human Sample Type: Serum Application: Neut Expression of heparin-binding epidermal growth factor-like growth factor and its receptors in the human fallopian tube and endometrium after treatment with mifepristone. Authors: Sun XX, Gemzell-Danielsson K, Li HZ, Stabi B, Stavreus-Evers A Fertil. Steril., 2006;85(1):171-8. Species: Human Sample Type: Whole Tissue Application: IHC Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor. Authors: Armant DR, Kilburn BA, Petkova A, Edwin SS, Duniec-Dmuchowski ZM, Edwards HJ, Romero R, Leach RE Development, 2006;133(4):751-9. Species: Human Sample Type: Whole Cells Application: ICC Trafficking of nuclear heparin-binding epidermal growth factor-like growth factor into an epidermal growth factor receptor-dependent autocrine loop in response to oxidative stress. Authors: Kim J, Adam RM, Freeman MR Cancer Res., 2005;65(18):8242-9. Species: Human Sample Type: Whole Cells Application: ICC Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK. Authors: Kansra S, Johnson JL Mol. Biol. Cell, 2004;15(9):4299-309. Species: Human Sample Type: Whole Cells Application: Neut Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype. Authors: Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR Dev. Biol., 2004;266(2):223-37. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HB-EGF (R&D Systems, Catalog # 259-HE) Asp63-Leu148 Accession # Q99075
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	HB-EGF was originally purified based on its heparin-binding property and mitogenic activity on BALB-3T3 fibroblasts from the conditioned medium of the human U-937 histiocytic lymphoma cell line. The natural protein has an apparent molecular mass of 19-23 kDa and exists in multiple forms as a result of heterogenous O-glycosylation and/or N-terminal truncation. In addition to fibroblasts, HB-EGF is also a potent mitogen for keratinocytes and smooth muscle cells but not for capillary endothelial cells. HB-EGF is produced in monocytes and macrophages. In addition, transcription of HB-EGF can be induced in vascular endothelial cells as well as aortic smooth muscle cells (SMC), suggesting that HB-EGF may have an important role in the pathogenesis of atherosclerosis. HB-EGF is a member of the EGF family of mitogens which also include transforming growth factor-alpha (TGF-alpha ), amphiregulin (AR), rat schwanoma-derived growth factor (SDGF), vaccinia growth factor (VGF), and the various ligands for the Her2/ErbB2/neu receptor. All these cytokines are derived from transmembrane precursors that contain one or several EGF structural units in their extracellular domain. Many of these transmembrane precursors are biologically active and seem to play a role in juxtacrine stimulation of adjacent cells. The cDNA for HB-EGF encodes a 204 amino acid residue transmembrane protein that is proteolytically cleaved to generate the soluble HB-EGF. Like EGF, TGF-alpha, and AR; HB-EGF binds to the EGF receptor and activates the receptor tyrosine kinase. HB-EGF is reported to be a more potent SMC mitogen than EGF. It has been suggested that the differential activities found for HB-EGF compared to EGF may be mediated by the heparin-binding properties of HB-EGF. A diphtheria toxin receptor that mediates the endocytosis of the bound toxin has been cloned and found to be identical to the transmembrane HB-EGF precursor.
运输条件	Blue Ice
存放说明	4℃
参考文献	EGF Family EGF Ligands

参考图片

HB-EGF in Human Placenta. HB-EGF was detected in immersion fixed paraffin-embedded sections of human placenta using 15 µg/mL Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑259‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Cell Proliferation Induced by HB‑EGF and Neutralization by Human HB‑EGF Antibody. Recombinant Human HB‑EGF (Catalog # 259-HE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HB‑EGF (20 ng/mL) is neutralized (green line) by increasing concentrations of Human HB‑EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA). The ND₅₀ is typically 0.1-0.6 µg/mL.

HB-EGF in Human Placenta. HB-EGF was detected in immersion fixed paraffin-embedded sections of human placenta using Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.