| 货号 | AF2589-SP |
| 别名 | artemin; ARTN; Enovin; EVN; EVNneurotrophic factor; NBN; Neublastin |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Artemin in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Artemin is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9048 (Human); 11876 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. ARTEMIN promotes de novo angiogenesis in ER negative mammary carcinoma through activation of TWIST1-VEGF-A signalling. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Artemin Ala108-Gly220 Accession # Q5T4W7 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human Artemin (ARTN; also known as enovin and neublastin) is a GDNF family ligand that is distantly related to the TGF-beta superfamily of molecules (1‑4). As such, it is synthesized as a preproprotein, and contains a variable length pre-, or signal sequence, plus a 68 amino acid (aa) proregion and a 113 aa mature segment (5‑7). Alternate splicing and start sites create signal sequences of 22, 30 and 39 aa, respectively. Their significance is unknown. Following synthesis and proteolytic processing, mature ARTN is secreted as a presumably glycosylated, 28 kDa disulfide-linked homodimer that contains three intrachain disulfide bonds and the typical TGF-beta signature cysteine-knot motif (5, 7). In the mature region, human ARTN is 89% and 88% aa identical to rat (8) and mouse ARTN (5, 7), respectively. Cells known to express ARTN include Schwann cells (2) and embryonic vascular smooth muscle cells (9). Human ARTN is active on rodent cells (5). The receptor for ARTN has been identified as the ligand binding subunit GFR alpha -3 plus the signal transducing subunit, RET (1, 5). The GFR alpha -1/RET receptor complex has also been suggested to be a ligand binding unit for ARTN (2, 5). Evidence, however, suggests that the GFR alpha -1/RET complex plays no functional role in ARTN activity (10, 11). ARTN is known to be a chemoattractant for sympathetic neuron axons innervating the developing cardiovascular system (9). It also promotes sensory neuron survival and likely plays a role in the development of the peripheral nervous system (5). Finally, it has been reported to reverse neuropathic pain due to nerve injury, and to help resolve morphological changes associated with nerve damage (12). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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