| 货号 | AF-257-SP |
| 别名 | ciliary neurotrophic factor; CNTF; HCNTF | 全称 | Ciliary Neurotrophic Factor |
| 反应种属 | Human |
| 应用 | Western Blot,Neutralization |
| 目标/特异性 | Detects human CNTF in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat CNTF is observed and less than 1% cross-reactivity with rhCNTF R alpha is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CNTF-induced proliferation in the TF‑1 human erythroleukemic cell line [Kitamura, T. et al. (1989) J. Cell Physiol. 140:323]. The Neutralization Dose (ND50) is typically 3-8 µg/mL in the presence of 400 ng/mL Recombinant Human CNTF. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1270 (Human); 25707 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Lentiviral-mediated transfer of CNTF to schwann cells within reconstructed peripheral nerve grafts enhances adult retinal ganglion cell survival and axonal regeneration. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CNTF (R&D Systems, Catalog # 257-NT) Ala2-Met200 Accession # P26441 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The gene for human CNTF has been localized to the proximal region of the long arm of chromosome 11. The cDNA for human CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by CNTF and Neutralization by Human CNTF Antibody. Recombinant Human CNTF (Catalog # 257-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CNTF (400 ng/mL) is neutralized (green line) by increasing concentrations of Human CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-257-NA). The ND50 is typically 3-8 µg/mL. |
