| 货号 | AF-252-SP |
| 别名 | HARP; HBBM; HB-GAM; HBGF8; HBNF; HBNF1; HBNF-1; Heparin-binding brain mitogen; HNGF-8; NEGF1HBGF-8; neurite growth-promoting factor 1; neurite growth-promoting factor1); OSF-1; Osteoblast-specific factor 1; Pleiotrophin; PTN |
| 反应种属 | Human |
| 应用 | Western Blot |
| 目标/特异性 | Detects human Pleiotrophin/PTN in direct ELISAs and Western blots. In direct ELISAs, less than 50% cross‑reactivity with recombinant mouse PTN is observed, and less than 1% cross‑reactivity with recombinant human Midkine is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5764 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A glucocorticoid- and diet-responsive pathway toggles adipocyte precursor cell activity in vivo. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Pleiotrophin/PTN Gly33-Asp168 Accession # P21246 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PTN was identified independently by several groups as a novel heparin-binding, developmentally regulated cytokine. Depending on the biological activities studied, this protein has variously been referred to as heparin-binding brain mitogen (HBBM), heparin-binding growth factor-8 (HBGF-8), heparin-binding neurite promoting factor, heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), heparin-binding growth-associated molecule (HB-GAM), PTN is a member of a family of heparin-binding proteins that share sequence, structural, and functional similarity. Other members of this family include midkine (MK), and chicken retinoic acid-induced heparin-binding protein (RI-HB), an avian homologue of MK. The expression of all these cytokines is restricted and highly regulated during development. PTN can be used as an attachment substrate to stimulate neurite outgrowth in mixed cultures of embryonic rat, mouse or chicken brain cells. Although both natural and recombinant human PTN have been reported to be mitogenic for fibroblasts, endothelial, and epithelial cells, the data are still highly controversial. PTN has been shown to transform NIH-3T3 and SW-13 cells, as evidenced by anchorage-independent growth and tumor formation in the nude mouse. These results suggest that, in spite of the conflicting reports of PTN’s growth-promoting activity in vitro, PTN may have a role in abnormal cell growth in vivo. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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