Human IL-15 R alpha Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human IL-15 R alpha Affinity Purified Polyclonal Ab (25 UG)

货号： AF247-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF247-SP
别名	CD215 antigen; CD215; IL-15 receptor subunit alpha; IL15RA; IL-15RA; IL-15R-alpha; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha; MGC104179
全称	Interleukin 15 Receptor alpha
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human IL-15 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human IL‑2 R gamma is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑15 R alpha -mediated inhibition of proliferation in the CTLL‑2 mouse cytotoxic T cell line. The Neutralization Dose (ND₅₀) is typically 1-4 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑15 R alpha Fc Chimera and 2 ng/mL Recombinant Human IL‑15.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3601 (Human); 16169 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells. Authors: Bergamaschi C, Kulkarni V, Rosati M, Alicea C, Jalah R, Chen S, Bear J, Sardesai N, Valentin A, Felber B, Pavlakis G Gene Ther, 2015;22(1):76-86. Species: Human Sample Type: Cell Lysates Application: WB Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways. Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D J Immunol, 2015;195(6):2818-28. Species: Human Sample Type: Whole Cells Application: ICC Fluorescence Regulation of CD4(+)NKG2D(+) Th1 cells in patients with metastatic melanoma treated with sorafenib: role of IL-15Ralpha and NKG2D triggering. Authors: Romero A, Chaput N, Poirier-Colame V, Rusakiewicz S, Jacquelot N, Chaba K, Mortier E, Jacques Y, Caillat-Zucman S, Flament C, Caignard A, Messaoudene M, Auperin A, Vielh P, Dessen P, Porta C, Mateus C, Ayyoub M, Valmori D, Eggermont A, Robert C, Zitvogel L Cancer Res, 2014;74(1):68-80. Species: Human Sample Type: tissue Application: IHC Paraffin-embedded Paracrine and transpresentation functions of IL-15 are mediated by diverse splice versions of IL-15Ralpha in human monocytes and dendritic cells. Authors: Muller J, Waldmann T, Kruhlak M, Dubois S J Biol Chem, 2012;287(48):40328-38. Species: Human Sample Type: Cell Lysates Application: ELISA Development Interleukin-15 plays a central role in human kidney physiology and cancer through the gamma c signaling pathway. Authors: Giron-Michel J, Azzi S, Khawam K, Mortier E, Caignard A, Devocelle A, Ferrini S, Croce M, Francois H, Lecru L, Charpentier B, Chouaib S, Azzarone B, Eid P PLoS ONE, 2012;7(2):e31624. Species: Human Sample Type: Cell Lysates Application: WB EBV-associated mononucleosis does not induce long-term global deficit in T-cell responsiveness to IL-15. Authors: Giron-Michel J, Menard F, Negrini S, Devocelle A, Azzarone B, Besson C Blood, 2009;113(19):4541-7. Species: Human Sample Type: Whole Cells Application: Flow Increased IL-15 production is associated with higher susceptibility of memory CD4 T cells to simian immunodeficiency virus during acute infection. Authors: Eberly MD, Kader M, Hassan W, Rogers KA, Zhou J, Mueller YM, Mattapallil MJ, Piatak M, Lifson JD, Katsikis PD, Roederer M, Villinger F, Mattapallil JJ J. Immunol., 2009;182(3):1439-48. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Cells Application: Flow Renal cells express a functional interleukin-15 receptor. Authors: Tejman-Yarden N, Zlotnik M, Lewis E, Etzion O, Chaimovitz C, Douvdevani A Nephrol. Dial. Transplant., 2005;20(3):516-23. Species: Human Sample Type: Cell Lysates Application: WB Human T cells constitutively express IL-15 that promotes ex vivo T cell homeostatic proliferation through autocrine/juxtacrine loops. Authors: Miranda-Carus ME, Benito-Miguel M, Llamas MA, Balsa A, Martin-Mola E J. Immunol., 2005;175(6):3656-62. Species: Human Sample Type: Whole Cells Application: Neut IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate. Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E J. Immunol., 2004;173(2):1463-76. Species: Human Sample Type: Whole Cells Application: Neut Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs. Authors: Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Munz C Proc. Natl. Acad. Sci. U.S.A., 2004;101(47):16606-11. Species: Human Sample Type: Whole Cells Application: Flow Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling. Authors: Yamasaki S, Maeda M, Ohshima K, Kikuchi M, Otsuka T, Harada M Leuk. Res., 2004;28(10):1023-31. Species: Human Sample Type: Whole Cells Application: Neut Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma. Authors: Hessian PA, Highton J, Kean A, Sun CK, Chin M Arthritis Rheum., 2003;48(2):334-8. Species: Human Sample Type: Whole Tissue Application: IHC
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑15 R alpha Ile31-Thr172 Accession # EAW86418
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms of IL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 amino acid (aa) protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain, and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma _c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.
运输条件	Blue Ice
存放说明	4℃
参考文献	Anderson, D.M.et al. (1995) J. Biol. Chem. 270:29862. Bulfone-Paus, S.et al. (1999) FASEB 13:1575. Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19. Dubois, S.et al. (1999) J. Biol. Chem. 274:26978.

参考图片

IL‑15 R alpha in Human Prostate Cancer Tissue. IL‑15 R alpha was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human IL‑15 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

IL‑15 R alpha Inhibition of IL‑15-dependent Cell Proliferation and Neutralization by Human IL‑15 R alpha Antibody. Recombinant Human IL‑15 R alpha Fc Chimera (Catalog #
147‑IR) inhibits Recombinant Human IL‑15 (Catalog #
247‑IL) induced proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑15 (2 ng/mL) activity elicited by Recombinant Human IL‑15 R alpha Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑15 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247). The ND₅₀ is typically
1‑4 µg/mL.