| 货号 | AF2430-SP |
| 别名 | Cancer/testis antigen 20; CT20probable threonine protease PRSS50; EC 3.4.25.-; protease, serine, 50; Serine protease 50; testes-specific protease 50; Testis-specific protease-like protein 50; TSP50testes specific protease 50 | 全称 | Testes-specific Protease 50 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human TSP50 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse TSP50 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 29122 (Human); 235631 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human TSP50 Ala40-Ala363 Accession # Q9UI38 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TSP50 is a member of the peptidase S1 family of serine proteases. It is a secreted glycoprotein that may play a role in spermatogenesis and act as an oncogene. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TSP50 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TSP50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2430) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TSP50 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
