Human TGF-beta RII Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human TGF-beta RII Affinity Purified Polyclonal Ab (25 UG)

货号： AF-241-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-241-SP
别名	AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II
全称	Transforming Growth Factor beta Receptor II
反应种属	Human
应用	Western Blot,Flow Cytometry,Immunohistochemistry,CyTOF-ready,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human TGF-beta RII in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) TGF‑ beta RII and less than 1% cross-reactivity with recombinant human TGF-beta RIII and rmTGF-beta RI is observed.
使用方法	Western Blot: 2 µg/mL Flow Cytometry: 1 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the TF‑1 human erythroleukemic cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND₅₀) is typically 5‑20 µg/mL in the presence of 0.04 ng/mL Recombinant Human TGF‑ beta 1 and 5 ng/mL Recombinant Human IL‑4.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7048 (Human); 21813 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. 24R,25-Dihydroxyvitamin D3 Protects against Articular Cartilage Damage following Anterior Cruciate Ligament Transection in Male Rats PLoS ONE, 2016;11(8):e0161782. Species: Rat Sample Type: Whole Cells Application: Blocking Clathrin light chains' role in selective endocytosis influences antibody isotype switching Proc Natl Acad Sci USA, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Bioassay Altered microenvironment promotes progression of preinvasive breast cancer: myoepithelial expression of alphavbeta6 integrin in DCIS identifies high-risk patients and predicts recurrence. Authors: Allen M, Thomas G, Clark S, Dawoud M, Vallath S, Payne S, Gomm J, Dreger S, Dickinson S, Edwards D, Pennington C, Sestak I, Cuzick J, Marshall J, Hart I, Jones J Clin Cancer Res, 2014;20(2):344-57. Species: Mouse Sample Type: Whole Cells Application: Neut CD4+CD25+ T regulatory cells activated during feline immunodeficiency virus infection convert T helper cells into functional suppressors through a membrane-bound TGFbeta / GARP-mediated mechanism. Authors: Miller M, Petty C, Tompkins M, Fogle J Virol J, 2014;11(0):7. Species: Feline Sample Type: Whole Cells Application: Neut Integrin-mediated type II TGF-beta receptor tyrosine dephosphorylation controls SMAD-dependent profibrotic signaling. Authors: Chen X, Wang H, Liao H, Hu W, Gewin L, Mernaugh G, Zhang S, Zhang Z, Vega-Montoto L, Vanacore R, Fassler R, Zent R, Pozzi A J Clin Invest, 2014;124(8):3295-310. Species: Mouse Sample Type: Cell Lysates Application: Neut T-cell TGF-beta signaling abrogation restricts medulloblastoma progression. Authors: Gate D, Danielpour M, Rodriguez J, Kim G, Levy R, Bannykh S, Breunig J, Kaech S, Flavell R, Town T Proc Natl Acad Sci U S A, 2014;111(33):E3458-66. Species: Human Sample Type: Whole Tissue Application: IHC Mesothelial cells promote early ovarian cancer metastasis through fibronectin secretion. Authors: Kenny H, Chiang C, White E, Schryver E, Habis M, Romero I, Ladanyi A, Penicka C, George J, Matlin K, Montag A, Wroblewski K, Yamada S, Mazar A, Bowtell D, Lengyel E J Clin Invest, 2014;124(10):4614-28. Species: Human Sample Type: Whole Cells Application: Neut TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells. Authors: Shinojima N, Hossain A, Takezaki T, Fueyo J, Gumin J, Gao F, Nwajei F, Marini F, Andreeff M, Kuratsu J, Lang F Cancer Res, 2013;73(7):2333-44. Species: Human Sample Type: Cell Lysates Application: WB Targeting of SMAD5 links microRNA-155 to the TGF-beta pathway and lymphomagenesis. Authors: Rai D, Kim SW, McKeller MR Proc. Natl. Acad. Sci. U.S.A., 2010;107(7):3111-6. Species: Human Sample Type: Whole Cells Application: Neut Antibody-mediated blockade of integrin alpha v beta 6 inhibits tumor progression in vivo by a transforming growth factor-beta-regulated mechanism. Authors: Van Aarsen LA, Leone DR, Ho S, Dolinski BM, McCoon PE, LePage DJ, Kelly R, Heaney G, Rayhorn P, Reid C, Simon KJ, Horan GS, Tao N, Gardner HA, Skelly MM, Gown AM, Thomas GJ, Weinreb PH, Fawell SE, Violette SM Cancer Res., 2008;68(2):561-70. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Tumor-induced anorexia and weight loss are mediated by the TGF-beta superfamily cytokine MIC-1. Authors: Johnen H, Lin S, Kuffner T, Brown DA, Tsai VW, Bauskin AR, Wu L, Pankhurst G, Jiang L, Junankar S, Hunter M, Fairlie WD, Lee NJ, Enriquez RF, Baldock PA, Corey E, Apple FS, Murakami MM, Lin EJ, Wang C, During MJ, Sainsbury A, Herzog H, Breit SN Nat. Med., 2007;13(11):1333-40. Species: Mouse Sample Type: In Vivo Application: In vivo Very low O2 concentration (0.1%) favors G0 return of dividing CD34+ cells. Authors: Hermitte F, Brunet de la Grange P, Belloc F, Praloran V, Ivanovic Z Stem Cells, 2005;24(1):65-73. Species: Human Sample Type: Whole Cells Application: Neut Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB Blood, 2005;105(2):750-8. Species: Human Sample Type: Cell Culture Supernates Application: Neut TGF-beta1 regulates TGF-beta1 and FGF-2 mRNA expression during fibroblast wound healing. Authors: Song QH, Klepeis VE, Trinkaus-Randall V MP, Mol. Pathol., 2002;55(3):164-76. Species: Rabbit Sample Type: Whole Tissue Application: IHC Transforming growth factor-beta1 regulation of growth zone chondrocytes is mediated by multiple interacting pathways. Authors: Rosado E, Schwartz Z, Sylvia VL, Dean DD, Boyan BD Biochim. Biophys. Acta, 2002;1590(1):1-15. Species: Rat Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta RII Ile24-Asp159 Accession # P37173.2
内毒素水平	<0.01 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	TGF beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF beta RII interacts with TGF beta RI to form the heteromeric signaling complex thattransduces TGF beta signals. A splice variant of the type II receptor, TGF beta RIIb, containing a 25 amino acid residue insertion near the Nterminus of the mature protein has also been described.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Cytokine and Growth Factor Receptors on VSMC Cytokines and Receptors in Angiogenesis Preeclampsia Regulatory T Cells (Tregs) T Cell Cytokine Signaling TGF-beta Family TGF-beta Superfamily Th17 Cells Th22 Cells Th9 Cells

参考图片

TGF-beta RII in Human Pituitary. TGF-beta RII was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of TGF-beta RII in Human Lymphocytes by Flow Cytometry. Human blood-derived lymphocytes were labeled with Goat Anti-Human TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF‑241‑NA, filled histogram) or control antibody (Catalog #
AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Detection of Human TGF‑ beta RII by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, HT1080 human fibrosarcoma cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TGF‑ beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TGF‑ beta RII at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by Human TGF‑ beta RII Antibody. Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Human IL‑4 (Catalog # 204-IL) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑4 (5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (0.04 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti- Human TGF‑ beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA). The ND₅₀ is typically
10‑20 µg/mL.