Human NCAM-1/CD56 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human NCAM-1/CD56 Affinity Purified Polyclonal Ab (25 UG)

货号： AF2408-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2408-SP
别名	CD56 / NCAM-1; CD56 antigen; CD56; MSK39; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM
全称	Neural Cell Adhesion Molecule
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human NCAM-1/CD56 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) ALCAM, rhBCAM and rhEpCAM is observed.
使用方法	Western Blot: 0.5 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4684 (Human); 17967 (Mouse); 24586 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Divergent modulation of neuronal differentiation by caspase-2 and -9. Authors: Pistritto G, Papaleo V, Sanchez P, Ceci C, Barbaccia ML PLoS ONE, 2012;7(5):e36002. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human NCAM1/CD56 Leu20-Pro603 Accession # NP_001070150
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca⁺⁺ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans (1), the extracellular matrix protein agrin (2), and several chondroitin sulfate proteoglycans that include neurocan and phosphocan (3). There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM-140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM-140 and NCAM-180 are type I transmembrane glycoproteins (4‑6). Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180 (7‑8). NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment (4). The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features (9). NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity (10‑13).
运输条件	Blue Ice
存放说明	4℃
参考文献	Burg, M.A. et al. (1995) J. Neurosci. Res. 41:49. Storms, S.D. and U. Rutishauser (1998) J Biol. Chem. 273:27124. Margolis, R.K. et al. (1996) Perspect. Dev. Neurobiol. 3:273. Dickson, G. et al. (1987) Cell 50:1119. Lanier, L.L. et al. (1991) J. Immunol. 146:4421. Hemperly, J.J. et al. (1990) J. Mol. Neurosci. 2:71. Rutishauser, U.and C. Goridis (1986) Trends Genet. 2:72. Vawter, M.P. et al. (2001) Exp. Neurol. 172:29. Rutihauser, U. (1990) Adv. Exp. Med. Biol. 265:179. Becker, C.G. et al. (1996) J. Neurosci. Res. 45:143. Doherty, P. et al. (1995) J. Neurobiol. 26:437. Eckardt, M. et al. (2000) J. Neurosci. 20:5234. Muller, D. et al. (1996) Neuron 17:413.

参考图片

NCAM-1/CD56 in SH-SY5Y Human Neuroblastoma Cells. SH-SY5Y human neuroblastoma cells were cultured overnight in the presence of 1 mM Retinoic Acid (Catalog # 0695/50) prior to immersion fixation. Neural Cell Adhesion Molecule 1 (NCAM-1)/CD56 was detected using a Goat Anti-Human NCAM-1/CD56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2408). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Goat IgG Affinity-purified Secondary Antibody (red; Catalog # NL001). Actin filaments were stained with FITC-conjugated Phalloidin (green) and cell nuclei were counterstained with DAPI (blue). NCAM-1/CD56 immunoreactivity was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

NCAM‑1/CD56 in BG01V Human Embryonic Stem Cells. NCAM‑1/CD56 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neural progenitor cells using Goat Anti-Human NCAM‑1/CD56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2408) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.