Human HNF-3 beta/FoxA2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human HNF-3 beta/FoxA2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF2400-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2400-SP
别名	forkhead box A2; Forkhead box protein A2; FoxA2; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta; hepatocyte nuclear factor 3-beta; HNF-3B; HNF-3-beta; HNF3BTCF-3B; MGC19807; TCF3B; Transcription factor 3B
全称	Hepatocyte Nuclear Factor-3, beta
反应种属	Human
应用	Western Blot(1 µg/mL) Chromatin Immunoprecipitation (ChIP)(5 µg/5 x 10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human HNF‑3 beta /FoxA2 in direct ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3170 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells Sci Rep, 2016;6(0):31063. Species: Mouse Sample Type: Whole Cells Application: IHC-Fluorescent A qPCR ScoreCard quantifies the differentiation potential of human pluripotent stem cells. Authors: Tsankov A, Akopian V, Pop R, Chetty S, Gifford C, Daheron L, Tsankova N, Meissner A Nat Biotechnol, 2015;33(11):1182-92. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates. Authors: Villa-Diaz L, Kim J, Lahann J, Krebsbach P Stem Cells Transl Med, 2014;3(12):1410-7. Species: Human Sample Type: Whole Cells Application: ICC Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells. Authors: Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L Cell Transplant, 2012;21(6):1321-39. Species: Human Sample Type: whole cells Application: ICC Fluorescence Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, oct4 and sox2. Authors: Montserrat N, Ramirez-Bajo MJ, Xia Y, Sancho-Martinez I, Moya-Rull D, Miquel-Serra L, Yang S, Nivet E, Cortina C, Gonzalez F, Izpisua Belmonte JC, Campistol JM J. Biol. Chem., 2012;287(29):24131-8. Species: Human Sample Type: Whole Cells Application: ICC Treatment with small molecules is an important milestone towards the induction of pluripotency in neural stem cells derived from human cord blood. Authors: Szablowska-Gadomska, Ilona, Sypecka, Joanna, Zayat, Valery, Podobinska, Martyna, Pastwinska, Anna, Pienkowska-Grela, Barbara, Buzanska, Leonora Acta Neurobiol Exp (Wars), 2012;72(4):337-50. Species: Human Sample Type: whole cells Application: ICC Complete meiosis from human induced pluripotent stem cells. Authors: Eguizabal C, Montserrat N, Vassena R, Barragan M, Garreta E, Garcia-Quevedo L, Vidal F, Giorgetti A, Veiga A, Belmonte JC Stem Cells, 2011;29(8):1186-95. Species: Human Sample Type: Whole Cells Application: ICC CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells. Authors: Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H Stem Cells, 2011;29(4):609-17. Species: Human Sample Type: Whole Cells Application: ICC A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3. Authors: Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ J. Cell. Sci., 2011;124(0):1992-2000. Species: Human Sample Type: Whole Cells Application: ICC Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm. Authors: Chayosumrit M, Tuch B, Sidhu K Biomaterials, 2010;31(3):505-14. Species: Human Sample Type: Cell Lysate Application: WB A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Authors: Klim JR, Li L, Wrighton PJ Nat. Methods, 2010;7(12):989-94. Species: Human Sample Type: Whole Cells Application: ICC Linking the p53 tumour suppressor pathway to somatic cell reprogramming. Authors: Kawamura T, Suzuki J, Wang YV, Menendez S, Morera LB, Raya A, Wahl GM, Belmonte JC Nature, 2009;460(7259):1140-4. Species: Mouse Sample Type: Whole Cells Application: ICC Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells. Authors: Zhang D, Jiang W, Liu M, Sui X, Yin X, Chen S, Shi Y, Deng H Cell Res., 2009;19(4):429-38. Species: Human Sample Type: Whole Cells Application: Flow Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells. Authors: Raya A, Rodriguez-Piza I, Guenechea G, Vassena R, Navarro S, Barrero MJ, Consiglio A, Castella M, RÃƒÂo P, Sleep E, Gonzalez F, Tiscornia G, Garreta E, Aasen T, Veiga A, Verma IM, Surralles J, Bueren J, Belmonte JC Nature, 2009;460(7251):53-9. Species: Human Sample Type: Whole Cells Application: ICC Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium. Authors: Nakanishi M, Kurisaki A, Hayashi Y, Warashina M, Ishiura S, Kusuda-Furue M, Asashima M FASEB J., 2009;23(1):114-22. Species: Human Sample Type: Whole Cells Application: ICC Generation of pluripotent stem cells from adult human testis. Authors: Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T Nature, 2008;456(7220):344-9. Species: Human Sample Type: Whole Cells Application: ICC Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells. Authors: Zhang P, Li J, Tan Z, Wang C, Liu T, Chen L, Yong J, Jiang W, Sun X, DU L, Ding M, Deng H Blood, 2007;111(4):1933-41. Species: Human Sample Type: Whole Cells Application: ICC Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: applications for the treatment of liver failure. Authors: Cho CH, Parashurama N, Park EY, Suganuma K, Nahmias Y, Park J, Tilles AW, Berthiaume F, Yarmush ML FASEB J., 2007;22(3):898-909. Species: Rat Sample Type: Whole Cells Application: Flow Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Authors: Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S Proc. Natl. Acad. Sci. U.S.A., 2006;103(18):6907-12. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human HNF‑3 beta /FoxA2 Met242-Ser457 Accession # Q9Y261
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	HNF-3 beta, also known as FoxA2, is a member of the forkhead class ofDNA-binding proteins. It is a transcriptional activator forliver-specific transcripts such as albumin and transthyretin. Similarfamily members play roles in the differentiation of the pancreas andliver.
运输条件	Blue Ice
存放说明	4℃
参考文献	Definitive Endoderm Markers Early Endodermal Lineage Markers Forkhead Domain Transcription Factors Hepatic and Pancreatic Progenitor Cell Transcription Factors Hepatic Endoderm Hepatic Endoderm Cell Markers Human Pluripotent Stem Cell Antibody Array ICC / IHC Images: HNF-3 beta/FoxA2 Transcription Factors and Regulators Transcription Factors in the Akt Pathway

参考图片

Detection of Human HNF‑3 beta /FoxA2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HNF‑3 beta /FoxA2 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of HNF‑3 beta /FoxA2-regulated Genes by Chromatin Immunoprecipitation. Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF‑3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The E-RABP promoter was detected by standard PCR.

HNF‑3 beta /FoxA2 in Endoderm Differentiated BG01V Human Stem Cells. HNF‑3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.