Human TGF-alpha Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human TGF-alpha Affinity Purified Polyclonal Ab (25 UG)

货号： AF-239-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-239-SP
别名	protransforming growth factor alpha; TFGA; TGFA; TGFalpha; TGF-alpha; transforming growth factor, alpha; transforming growth factor-alpha
全称	Transforming Growth Factor alpha
反应种属	Human
应用	Western Blot,Immunohistochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human TGF-alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with recombinant human (rh) TGF‑ beta 1, rhAmphiregulin, rhBetacellulin, rhEGF, rhHRG-alpha, and rhSMDF is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize TGF‑ alpha -induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.15-0.75 μg/mL in the presence of 3 ng/mL Recombinant Human TGF‑ alpha.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7039 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Temporal expression of growth factors triggered by epiregulin regulates inflammation development. Authors: Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M J Immunol, 2015;194(3):1039-46. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Amphiregulin carboxy-terminal domain is required for autocrine keratinocyte growth. Authors: Stoll SW, Johnson JL, Li Y, Rittie L, Elder JT J. Invest. Dermatol., 2010;130(8):2031-40. Species: Human Sample Type: Whole Cells Application: Neut Association of tetraspanin CD9 with transmembrane TGFalpha confers alterations in cell-surface presentation of TGFalpha and cytoskeletal organization. Authors: Imhof I, Gasper WJ, Derynck R J. Cell. Sci., 2008;121(0):2265-74. Species: Human Sample Type: Whole Cells Application: IP The luteinizing hormone receptor-activated extracellularly regulated kinase-1/2 cascade stimulates epiregulin release from granulosa cells. Authors: Andric N, Ascoli M, Endocrinology, 2008;149(11):5549-56. Species: Mouse Sample Type: Whole Cells Application: Neut Development and validation of sandwich ELISA microarrays with minimal assay interference. Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA J. Proteome Res., 2008;7(6):2406-14. Species: Human Sample Type: Serum Application: ELISA Microarray Development EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells. Authors: Liu M, Yang SC, Sharma S, Luo J, Cui X, Peebles KA, Huang M, Sato M, Ramirez RD, Shay JW, Minna JD, Dubinett SM Am. J. Respir. Cell Mol. Biol., 2007;37(5):578-88. Species: Human Sample Type: Whole Cells Application: Neut Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells. Authors: Wolff GS, Chiang PJ, Smith SM, Romero R, Armant DR Biol. Reprod., 2007;77(1):53-60. Species: Human Sample Type: Whole Cells Application: ICC Expression and role of EGFR ligands induced in airway cells by PM2.5 and its components. Authors: Rumelhard M, Ramgolam K, Hamel R, Marano F, Baeza-Squiban A Eur. Respir. J., 2007;30(6):1064-73. Species: Human Sample Type: Whole Cells Application: Neut The response of human epithelial cells to TNF involves an inducible autocrine cascade. Authors: Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK Cell, 2006;124(6):1225-39. Species: Human Sample Type: Whole Cells Application: Neut Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration. Authors: Li Y, Fan J, Chen M, Li W, Woodley DT J. Invest. Dermatol., 2006;126(9):2096-105. Species: Human Sample Type: Serum Application: Immunodepletion Mek1 alters epidermal growth and differentiation. Authors: Scholl FA, Dumesic PA Cancer Res., 2004;64(17):6035-40. Species: Mouse Sample Type: Tissue Homogenates Application: WB Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype. Authors: Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR Dev. Biol., 2004;266(2):223-37. Species: Human Sample Type: Whole Cells Application: ICC Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK. Authors: Kansra S, Johnson JL Mol. Biol. Cell, 2004;15(9):4299-309. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human TGF-alpha Val40-Ala89 Accession # P01135
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	TGF-alpha was originally isolated from the conditioned media of oncogenically transformed cells as an EGF-like bioactivity. TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released from the transmembrane protein by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and seems to play a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. Mature TGF-alpha shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects. TGF-alpha binds to the EGF receptor and activates the receptor tyrosine kinase. Accordingly, TGF-alpha shows a similar potency to EGF as a mitogen for fibroblasts and as an inducer of epithelial development in vivo. TGF-alpha is reportedly more potent than EGF as an angiogenic factor in vivo and as a stimulator for keratinocyte migration. The EGF receptor gene represents the cellular homologue of the avian v-erb-B oncogene.
运输条件	Blue Ice
存放说明	4℃
参考文献	EGF Family EGF Ligands HIF Transcription Factors

参考图片

TGF‑ alpha in Human Astrocytoma. TGF‑ alpha was detected in immersion fixed paraffin-embedded sections of human astrocytoma using 15 µg/mL Goat Anti-Human TGF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑239‑NA) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Cell Proliferation Induced by TGF‑ alpha and Neutralization by Human TGF‑ alpha Antibody.

Recombinant Human TGF‑ alpha (Catalog # 239-A) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TGF‑ alpha (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TGF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-239-NA). The ND₅₀ is typically 0.15‑0.75 μg/mL.

TGF-alpha in Human Astrocytoma. TGF-alpha was detected in immersion fixed paraffin-embedded sections of human astrocytoma using Goat Anti-Human TGF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-239-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.