Human EGF Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human EGF Affinity Purified Polyclonal Ab (25 UG)

货号： AF236-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF236-SP
别名	beta-urogastrone; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone
全称	Epidermal Growth Factor
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human EGF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat EGF is observed, 10% cross-reactivity with recombinant mouse EGF, and less than 1% cross-reactivity with recombinant human (rh) AR, rhBTC, rhHB-EGF, rhHRG-alpha, or rhHRG1-beta 1 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.04-0.08 µg/mL in the presence of 2 ng/mL Recombinant Human EGF.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1950 (Human); 13645 (Mouse); 25313 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK J. Immunol. Methods, 2012;378(1):72-80. Species: Human Sample Type: Serum Application: ELISA Development EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells. Authors: Liu M, Yang SC, Sharma S, Luo J, Cui X, Peebles KA, Huang M, Sato M, Ramirez RD, Shay JW, Minna JD, Dubinett SM Am. J. Respir. Cell Mol. Biol., 2007;37(5):578-88. Species: Human Sample Type: Whole Cells Application: Neut Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells. Authors: Wolff GS, Chiang PJ, Smith SM, Romero R, Armant DR Biol. Reprod., 2007;77(1):53-60. Species: Human Sample Type: Whole Cells Application: ICC Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells. Authors: Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK Invest. Ophthalmol. Vis. Sci., 2006;47(3):864-72. Species: Human Sample Type: Cell Lysates Application: WB Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration. Authors: Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG Eur J Vasc Endovasc Surg, 2006;31(5):516-22. Species: Human Sample Type: Whole Tissue Application: IHC Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype. Authors: Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR Dev. Biol., 2004;266(2):223-37. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human EGF
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers Cytokines and Receptors in Angiogenesis EGF Family EGF Ligands EMT Induction gamma delta T Cells ICC / IHC Images: EGF Inflammatory Mediators TrkB Integrates Neural Precursor Cell Migration & Differentiation in the Developing Brain

参考图片

Cell Proliferation Induced by EGF and Neutralization by Human EGF Antibody. Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236). The ND₅₀ is typically 0.04-0.08 µg/mL.

EGF in Human Placenta. EGF was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.