Human FGF basic Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human FGF basic Affinity Purified Polyclonal Ab (25 UG)

货号： AF-233-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-233-SP
别名	basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGF2AS; FGFBprostatropin; fibroblast growth factor 2 (basic); GFG1; HBGF-2; HBGH-2; heparin-binding growth factor 2; NUDT6; Prostatropin
全称	Fibroblast Growth Factor basic
反应种属	Human
应用	Western Blot,Immunohistochemistry,Neutralization
目标/特异性	Detects human and bovine FGF basic in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant mouse FGF basic is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize FGF basic-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND₅₀) is typically 0.08-0.4 µg/mL in the presence of 0.5 ng/mL Bovine FGF basic.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Endothelial heparan sulfate 6-o-sulfation levels regulate angiogenic responses of endothelial cells to fibroblast growth factor 2 and vascular endothelial growth factor. Authors: Ferreras, Cristina, Rushton, Graham, Cole, Claire L, Babur, Muhammad, Telfer, Brian A, van Kuppevelt, Toin H, Gardiner, John M, Williams, Kaye J, Jayson, Gordon C, Avizienyte, Egle J Biol Chem, 2102;287(43):36132-46. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Pericytes impair capillary blood flow and motor function after chronic spinal cord injury Authors: Y Li, AM Lucas-Osma, S Black, MV Bandet, MJ Stephens, R Vavrek, L Sanelli, KK Fenrich, AF Di Narzo, S Dracheva, IR Winship, K Fouad, DJ Bennett Nat. Med., 2017;0(0):. Species: Rat Sample Type: Whole Tissue Application: IHC Tumor-associated B-cells induce tumor heterogeneity and therapy resistance Authors: R Somasundar, G Zhang, M Fukunaga-K, M Perego, C Krepler, X Xu, C Wagner, D Hristova, J Zhang, T Tian, Z Wei, Q Liu, K Garg, J Griss, R Hards, M Maurer, C Hafner, M Mayerhöfer, G Karanikas, A Jalili, V Bauer-Pohl, F Weihsengru, K Rappersber, J Koller, R Lang, C Hudgens, G Chen, M Tetzlaff, L Wu, DT Frederick, RA Scolyer, GV Long, M Damle, C Ellingswor, L Grinman, H Choi, BJ Gavin, M Dunagin, A Raj, N Scholler, L Gross, M Beqiri, K Bennett, I Watson, H Schaider, MA Davies, J Wargo, BJ Czerniecki, L Schuchter, D Herlyn, K Flaherty, M Herlyn, SN Wagner Nat Commun, 2017;8(1):607. Species: Human Sample Type: Whole Cells Application: Neut Peroxynitrite upregulates angiogenic factors VEGF-A, BFGF, and HIF-1alpha in human corneal limbal epithelial cells. Authors: Ashki N, Chan A, Qin Y, Wang W, Kiyohara M, Lin L, Braun J, Wadehra M, Gordon L Invest Ophthalmol Vis Sci, 2014;55(3):1637-46. Species: Human Sample Type: Cell Lysates Application: WB Inhibition of PAI-1 induces neutrophil-driven neoangiogenesis and promotes tissue regeneration via production of angiocrine factors in mice. Blood, 2012;119(26):6382-93. Species: Mouse Sample Type: In Vivo Application: In Vivo The fibroblast-derived paracrine factor neuregulin-1 has a novel role in regulating the constitutive color and melanocyte function in human skin. Authors: Choi W, Wolber R, Gerwat W J. Cell. Sci., 2010;123(0):3102-11. Species: Human Sample Type: Cell Lysates Application: WB Pancreatic stellate cells: partners in crime with pancreatic cancer cells. Authors: Vonlaufen A, Joshi S, Qu C, Phillips PA, Xu Z, Parker NR, Toi CS, Pirola RC, Wilson JS, Goldstein D, Apte MV Cancer Res., 2008;68(7):2085-93. Species: Human Sample Type: Whole Cells Application: Neut Temporally expressed PDGF and FGF-2 regulate embryonic coronary artery formation and growth. Authors: Tomanek RJ, Hansen HK, Christensen LP Arterioscler. Thromb. Vasc. Biol., 2008;28(7):1237-43. Species: Avian Sample Type: Whole Tissue Application: Neut A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells. Authors: Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC Exp. Cell Res., 2008;314(11):2176-86. Species: Mouse Sample Type: Whole Cells Application: Neut IFATS Series: FGF-2-induced HGF Secretion By Adipose-Derived Stromal Cells Inhibits Post-Injury Fibrogenesis Through A JNK-Dependent Mechanism. Authors: Suga H, Eto H, Shigeura T, Inoue K, Aoi N, Kato H, Nishimura S, Manabe I, Gonda K, Yoshimura K Stem Cells, 2008;0(0):. Species: Mouse Sample Type: In Vivo Application: In vivo Agrin, a novel basement membrane component in human and rat liver, accumulates in cirrhosis and hepatocellular carcinoma. Authors: Tatrai P, Dudas J, Batmunkh E, MÃƒÂ¡thÃƒÂ© M, Zalatnai A, Schaff Z, Ramadori G, Kovalszky I Lab. Invest., 2006;86(11):1149-60. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Positive correlation between estradiol and vascular endothelial growth factor but not fibroblast growth factor-2 in normal human breast tissue in vivo. Authors: Dabrosin C Clin. Cancer Res., 2005;11(22):8036-41. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis. Authors: Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP Development, 2005;132(6):1223-34. Species: Mouse Sample Type: Whole Cells Application: Neut Differential role of platelet granular mediators in angiogenesis. Authors: Brill A, Elinav H, Varon D Cardiovasc. Res., 2004;63(2):226-35. Species: Bovine Sample Type: Whole Cells Application: Neut Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis. Authors: Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V Blood, 2003;102(2):638-45. Species: Human Sample Type: Whole Cells Application: Neut The anti-HIV cytokine midkine binds the cell surface-expressed nucleolin as a low affinity receptor. Authors: Said EA, Krust B, Nisole S, Svab J, Briand JP, Hovanessian AG J. Biol. Chem., 2002;277(40):37492-502. Species: Hamster Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Bovine brain-derived FGF basic
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35 ‑ 60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. A variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. FGF basic induces neuron differentiation, survival, and regeneration. FGF basic also modulates embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.
运输条件	Blue Ice
存放说明	4℃
参考文献	Coulier, F. et al. (1997) J. Mol. Evol. 44:43. Chen, C.H. et al. (2004) Curr. Vasc. Pharmacol. 2:33. Mohammadi, M. et al. (2005) Curr. Opin. Struct. Biol. 15:506. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.

参考图片

FGF basic in Human Placenta. FGF basic was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human FGF basic Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑233‑NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Cell Proliferation Induced by FGF basic and Neutralization by Human FGF basic Antibody. Bovine FGF basic (Catalog # 133-FB) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF basic (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF basic Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-233-NA). The ND₅₀ is typically 0.08‑0.4 µg/mL.