Human Aminopeptidase PILS Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human Aminopeptidase PILS Affinity Purified Polyclonal Ab (25 UG)

货号： AF2334-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF2334-SP
别名	Adipocyte-derived leucine aminopeptidase; A-LAP; A-LAPALAP; Aminopeptidase PILS; ARTS1; ARTS1aminopeptidase regulator of TNFR1 shedding; ARTS-1PILSAP; EC 3.4.11; EC 3.4.11.-; EC 3.4.11.1; endoplasmic reticulum aminopeptidase 1; endoplasmic reticulum aminopeptidase associated with antigen processing; ERAAP1; ERAP1; KIAA0525APPILS; PILS-AP; PILS-APERAAP; Puromycin-insensitive leucyl-specific aminopeptidase; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator
全称	Aminopeptidase Puromycin-insensitive Leucyl-specific
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(1-15 µg/mL) Immunoprecipitation(25 µg/mL) Immunocytochemistry(1-15 µg/mL)
目标/特异性	Detects human Aminopeptidase PILS/ARTS1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross‑reactivity with recombinant mouse ARTS1 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 1-15 µg/mL Immunoprecipitation: 25 µg/mL Immunocytochemistry: 1-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	51752 (Human); 80898 (Mouse)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase PILS/ARTS1 Ala37-Met941 Accession # EAW96077
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The name of Aminopetidase PILS (Puromycin-Insensitive Leucyl-Specific) describes the two basic properties of this zinc metalloprotease in vitro(1). Also known as ALAP (Adipocyte-derived Leucin AiminoPeptidase), type 1 tumor necrosis factor receptor (TNFR) shedding aminopeptidase regulator and ER aminopeptidase ERAP1 or ERAAP, it is encoded by the ARTS1 gene (2‑4). Aminopeptidase PILS has been identified to regulate antigen presentation, promote TNFR1 ectodomain shedding and associate with hypertension (2‑5).
运输条件	Blue Ice
存放说明	4℃
参考文献	Schomburg, L. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 311, Academic Press, San Diego. Cui, X. et al. (2002) J. Clin. Invest. 110:515. York, I.A. et al. (2002) Nat. Immunol. 3:1177. Serwold, T. et al. (2002) Nature 419:480. Yamamoto, N. et al. (2002) Hum. Mutat. 19:251.

参考图片

Aminopeptidase PILS/ARTS1 in A549 Human Cell Line. Aminopeptidase PILS/ARTS1 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Aminopeptidase PILS/ARTS1 in Human Liver. Aminopeptidase PILS/ARTS1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.