| 货号 | AF2320-SP |
| 别名 | IL-1 epsilon; IL-1 H1; IL-1 Related Protein 2; IL-1(EPSILON); IL1E; IL-1-epsilon; IL1F9; IL-1F9; IL1H1; IL-1H1; IL-1-Related Protein 2; IL1RP2; IL-1rp2; IL36G; interleukin 1 family, member 9; interleukin 1-related protein 2; Interleukin 36, Gamma; Interleukin-1 epsilon; interleukin-1 family member 9; Interleukin-1 homolog 1; Interleukin-36 Gamma | 全称 | Interleukin 36 gamma/Interleukin 1 Family 9 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human IL‑36 gamma /IL‑1F9 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) IL-36 alpha is observed, approximately 5% cross-reactivity with rhIL-37 is observed, and less than 1% cross-reactivity with rhIL-1 alpha, rhIL-1 beta, rhIL‑36Ra, and rhIL‑36 beta is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.7-2.8 µg/mL of this antibody will block 50% of the binding of 5 μg/mL of Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera (Catalog # 872-RP) to immobilized Recombinant Human IL‑36 gamma /IL‑1F9 (Catalog # 2320-IL) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 56300 (Human); 215257 (Mouse); 499744 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The double-stranded RNA analogue polyinosinic-polycytidylic acid induces keratinocyte pyroptosis and release of IL-36 gamma. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human IL‑36 gamma /IL‑1F9 (R&D Systems, Catalog # 2320-IL) Met1-Asp169 Accession # Q9NZH8 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human interleukin 1 family member #9 [IL-1F9; also named Interleukin-36 gamma, IL36G, IL-1 epsilon (epsilon) and IL-1H1] is a member of the IL-1 family, which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (1‑4). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-1F9 is synthesized as a 19 kDa, 169 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site (1, 2, 5). The molecule is secreted when transfected into 293-T cells (5). Human to mouse, IL-1F9 has 53% aa sequence identity. Within the family, IL-1F9 shares 30%, 23%, 33%, 57%, 35%, 45%, and 32% aa sequence identity with IL-1 ra, IL-1 beta, IL-1F5, F6, F7, F8, and F10, respectively. Cells reported to express IL-1F9 include Langerhans cells, keratinocytes/stratified squamous epithelium, plus Chief cells and Parietal cells of the stomach (4, 5). The receptor for IL‑1F9 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (6). Recombinant IL-1F9, along with IL-1F8 and IL-1F6, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human IL‑36 gamma /IL‑1F9 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human IFN‑ gamma (Catalog # 285‑IF) and 20 ng/mL Recombinant Human TNF‑ alpha (Catalog # 210‑TA) for 6 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL‑36 gamma /IL‑1F9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2320) followed by HRP‑conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑36 gamma /IL‑1F9 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
