| 货号 | AF-230-SP |
| 别名 | CD124 antigen; CD124; IL-4 R alpha; IL-4 receptor subunit alpha; IL4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124; IL-4R-alpha; interleukin 4 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha | 全称 | Interleukin 4 Receptor alpha |
| 反应种属 | Human |
| 应用 | Western Blot,Flow Cytometry,CyTOF-ready |
| 目标/特异性 | Detects human IL-4 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) IL-1 sRI, rhIL-1 sRII, rhIL-2 sR alpha, rhIL-2 sR beta, rhIL-2 sR gamma, rhIL-3 sR alpha, and rhIL-5 sR alpha is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3566 (Human); 16190 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Opposing control of rhabdomyosarcoma growth and differentiation by myogenin and interleukin 4. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑4 R alpha |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interleukin 4 (IL‑4) is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. The biological functions of IL‑4 are mediated by the binding of IL‑4 to high‑affinity cell surface receptor complexes. Two types of IL‑4 receptor complexes have been described. The type I IL‑4 receptor complex is composed of a high‑affinity IL‑4‑binding subunit (referred to as IL‑4 R alpha ) and the common gamma chain that does not bind IL‑4 by itself. The type II IL‑4 receptor complex is composed of IL‑4 R alpha and IL‑13 R alpha 1. Besides IL‑4 signals, the type II IL‑4 receptor complex can also transduce IL‑13 signals. In the type II complex, the IL‑4 R alpha subunit binds only IL‑4 and not IL‑13. Similarly, the IL‑13 R alpha 1 subunit binds only IL‑13 and not IL‑4. The cDNA clones for both the human and mouse IL‑4 R alpha have been isolated and shown to encode an approximately 140 kDa type I transmembrane protein with a large cytoplasmic domain that is essential for signal transduction. In mouse cells, an alternatively spliced variant encoding a soluble secreted IL‑4 R alpha isoform has also been identified. Naturally occurring soluble IL‑4 R alpha that binds IL‑4 with high‑affinity has been found in mouse and human biological fluids. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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