Human gp130 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human gp130 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-228-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-228-SP
别名	CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha
全称	Glycoprotein 130
反应种属	Human
应用	Western Blot,Neutralization
目标/特异性	Detects human gp130 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant rat gp130, recombinant mouse gp130, recombinant human (rh) IL-1 sRI, rhIL-1 sRII, rhIL-2 sR beta, rhIL-2 sR gamma, rhIL‑4 sR, and rhIL-6 sR is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 2‑10 µg/mL in the presence of 0.8 ng/mL Recombinant Human Oncostatin M/OSM.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3572 (Human); 16195 (Mouse); 25205 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. NF-kappaB-induced IL-6 ensures STAT3 activation and tumor aggressiveness in glioblastoma. Authors: McFarland B, Hong S, Rajbhandari R, Twitty G, Gray G, Yu H, Benveniste E, Nozell S PLoS ONE, 2013;8(11):e78728. Species: Human Sample Type: Whole Cells Application: Neut Activation of the AXL kinase causes resistance to EGFR-targeted therapy in lung cancer. Authors: Zhang Z, Lee JC Nat. Genet., 2012;44(8):852-860. Species: Human Sample Type: Cell Lysates Application: WB Disturbances in the LIF pathway in the endometrium among women with unexplained infertility. Authors: Aghajanova L, Altmae S, Bjuresten K, Hovatta O, Landgren BM, Stavreus-Evers A Fertil. Steril., 2009;91(6):2602-10. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells. Authors: Aghajanova L, Skottman H, Stromberg AM, Inzunza J, Lahesmaa R, Hovatta O Fertil. Steril., 2006;86(4):1193-209. Species: Human Sample Type: Whole Cells Application: ICC Breast cancer cells stimulate neutrophils to produce oncostatin M: potential implications for tumor progression. Authors: Queen MM, Ryan RE, Holzer RG, Keller-Peck CR, Jorcyk CL Cancer Res., 2005;65(19):8896-904. Species: Human Sample Type: Whole Cells Application: Neut The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling. Authors: He W, Gong K, Smith DK, Ip NY FEBS Lett., 2005;579(20):4317-23. Species: Human Sample Type: Cell Lysates Application: WB Alterations in Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in patients with end-stage dilated cardiomyopathy. Authors: Podewski EK, Hilfiker-Kleiner D, Hilfiker A, Morawietz H, Lichtenberg A, Wollert KC, Drexler H Circulation, 2003;107(6):798-802. Species: Human Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human gp130 Leu24-Glu619 (Glu619Asp) Accession # P40189
内毒素水平	<0.70 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R. Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. At the present time, the in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.
运输条件	Blue Ice
存放说明	4℃
参考文献	Narazaki, M.et al. (1993) Blood 82:1120. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.

参考图片

Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody. Recombinant Human Oncostatin M/OSM (Catalog # 295-OM) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Oncostatin M/OSM (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human gp130 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-228-NA). The ND₅₀ is typically 2‑10 µg/mL.