| 货号 | AF-227-SP |
| 别名 | CD126; IL-6 R alpha; IL6R alpha; IL6R; IL-6Ra | 全称 | Interleukin 6 Receptor alpha |
| 反应种属 | Human |
| 应用 | Western Blot,Neutralization |
| 目标/特异性 | Detects human IL-6 R alpha in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑6 R alpha -mediated inhibition of proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 0.2-0.4 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑6 R alpha and 30 ng/mL Recombinant Human IL‑6. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3570 (Human); 16194 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. An autoinflammatory neurological disease due to interleukin 6 hypersecretion. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑6 R alpha Leu20-Asp358 Accession # P08887 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4). A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Recombinant Human IL‑6 R alpha by Western Blot. Western blot shows 5 ng of Recombinant Human IL‑6 R alpha (Catalog # 227-SR), Recombinant Mouse IL‑6 R alpha (Catalog # 1830-SR) and Recombinant Rat IL-6 R. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL‑6 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑6 R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. | |
IL‑6 R alpha Enhancement of IL‑6‑dependent Inhibition of Cell Proliferation and Neutralization by Human IL‑6 R alpha Antibody. Recombinant Human IL‑6 R alpha (Catalog # 227‑SR) enhances Recombinant Human IL‑6 (Catalog # |
