Human TNF RI/TNFRSF1A Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human TNF RI/TNFRSF1A Affinity Purified Polyclonal Ab (25 UG)

货号： AF225-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF225-SP
别名	CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
全称	Tumor Necrosis Factor Receptor I
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human sTNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 10% cross-reactivity with recombinant mouse TNF sRI is observed. Additionally, in direct ELISAs, this antibody does not cross-react with recombinant human (rh) sTNF RII, recombinant mouse (rm) sTNF RII, rhTNF‑ alpha, rmTNF‑ alpha, rhTNF‑ beta. This anti-human TNF RI antibody can interact with the cell surface 55 kDa human TNF RI and exhibits TNF agonist activities on the A549 human lung carcinoma cell line. This antibody also exhibits TNF agonist activities on the L‑929 mouse fibroblast cell line, suggesting that it has cross-species activity with mouse cell surface TNF RI.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Agonist Activity: Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is typically 1-6 μg/mL. Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7132 (Human); 21937 (Mouse); 25625 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based Vaccine. Authors: Luci C, Bekri S, Bihl F, Pini J, Bourdely P, Nouhen K, Malgogne A, Walzer T, Braud V, Anjuere F PLoS ONE, 2015;10(12):e0143224. Species: Mouse Sample Type: Whole Cells Application: IHC-Frozen Ubiquitin-associated domain-containing ubiquitin regulatory X (UBX) protein UBXN1 is a negative regulator of nuclear factor kappaB (NF-kappaB) signaling. Authors: Wang Y, Tan B, Mu R, Chang Y, Wu M, Tu H, Zhang Y, Guo S, Qin X, Li T, Li W, Li A, Zhang X, Li H J Biol Chem, 2015;290(16):10395-405. Species: Human Sample Type: Whole Cells Application: IP Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK J. Immunol. Methods, 2012;378(1):72-80. Species: Human Sample Type: Serum Application: ELISA Development Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expression. Authors: Hadjadj S, Cloutier I, Geoffroy P, Tanguay JF Atherosclerosis, 2007;192(1):25-32. Species: Porcine Sample Type: Whole Tissue Application: IHC Paraffin-embedded Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers. Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH Dig. Dis. Sci., 2007;52(10):2775-83. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human sTNF RI/TNFRSF1A Accession # NP_001056
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is typically 1-6 μg/mL.
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adipocytokines Cell Structure and Signaling Molecules Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines in Lipid/Cholesterol Metabolism Diabetes Diabetic Peripheral Neuropathy EMT Induction Endothelial Cell Markers Human CD Marker Products ICC / IHC Images: TNF RI/TNFRSF1A Neuroinflammation Obesity Regulation of Apoptosis by TNF Superfamily Members T Cell Cytokine Signaling TNF Superfamily Receptors

参考图片

TNF RI/TNFRSF1A in Human Peripheral Blood Lymphocytes. TNF RI/TNFRSF1A was detected in immersion fixed human peripheral blood lymphocytes using 5 µg/mL Human TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF225) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.