| 货号 | AF2249-SP |
| 别名 | CD336 antigen; CD336; LY95; LY95lymphocyte antigen 95 homolog (activating NK-receptor; NK-p44); lymphocyte antigen 95 (activating NK-receptor; NK-p44); Lymphocyte antigen 95 homolog; natural cytotoxicity triggering receptor 2; Natural killer cell p44-related protein; NCR2; NK cell activating receptor (NKp44); NK cell-activating receptor; NKP44; NK-p44dJ149M18.1 | 全称 | NK Cell Activating Receptor |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Agonist Activity(0.2-0.6 µg/mL) |
| 目标/特异性 | Detects human NKp44/NCR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) NKp30, rhNKp46, and rhNKp80 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Agonist Activity: 0.2-0.6 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9436 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human NKp44/NCR2 Gln22-Pro190, predicted Accession # O95944 |
| 内毒素水平 | <1.5 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | NKp44, along with NKp30 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors" (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering NK-mediated killing of most tumor cell lines. No rodent ortholog to NKp44 has been identified. Human NKp44, also known as NCR2, is a 44 kDa type I transmembrane glycoprotein that is characterized by the presence of one extracellular V-like immunoglobulin domain (2). It is synthesized as a 276 amino acid (aa) precursor that contains a 21 aa signal sequence, a 171 aa extracellular region, a 21 aa transmembrane segment and a 63 aa cytoplasmic tail. Alternate splicing in both the cytoplasmic tail and extracellular region generates multiple isoforms of unknown significance. The Ig-like region is unaffected. A physical association with the ITAM-bearing accessory protein, DAP12, occurs via a charged residue in the NKp44 transmembrane domain. Ligation of NKp44 with a specific antibody results in phosphorylation of DAP12 (3) and activation of target cell lysis in a redirected killing assay (4). NKp44 is absent from resting NK cells but is up‑regulated upon activation with IL-2. Activation-induced expression occurs in the CD56dimCD16+ NK subset that accounts for more than 85% of NK cells found in peripheral blood and spleen, as well as the CD56brightCD16- NK subset that constitutes the majority of NK cells in lymph node and tonsil (5). Studies with neutralizing antibodies reveal that NKp44 is partially responsible for triggering lytic activity against several tumor cell types (2, 6). Blocking any of the individual NCRs results in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis is observed if all three receptors are blocked simultaneously (6). NKp44 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (7). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Human NKp44 Antibody Induces IFN-gamma Secretion in Human NK Cells. Human NKp44 Antigen Affinity-purified Polyclonal Antibody induces IFN-gamma secretion in human natural killer (NK) cells activated with 1,000 U/mL Recombinant Human IL-2 (Catalog # 202-IL), in a dose-dependent manner, as measured using the Quantikine Human IFN‑ gamma ELISA Kit (Catalog # DIF50). The ED50for this effect is typically 0.2‑0.6 μg/mL. |
